Tested Applications
| Positive WB detected in | HeLa cells, rat testis tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16039-1-AP targets TUSC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8912 Product name: Recombinant human TUSC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 45-199 aa of BC010370 Sequence: KENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVCRQANEEYQILANSWRYSSAFCNKLFFSMVDYDEGTDVFQQLNMNSAPTFMHFPPKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYS Predict reactive species |
| Full Name | tumor suppressor candidate 3 |
| Calculated Molecular Weight | 347 aa, 40 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC010370 |
| Gene Symbol | TUSC3 |
| Gene ID (NCBI) | 7991 |
| RRID | AB_2878208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13454 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TUSC3 antibody 16039-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TUSC3 Polyclonal antibody (Cat# 16039-1-AP) has 11 citations published in journals including Nature Communications, PNAS, Journal of Allergy and Clinical Immunology, Journal of Translational Medicine, Journal of Cell Biology, Scientific Reports, BMC Cancer, Journal of Biological Chemistry, Glycobiology, and Frontiers in Genetics. Research fields span cancer biology (NSCLC metastasis, hepatocellular carcinoma, esophageal cancer, cholangiocarcinoma), neurodevelopment and synaptic function, immunology (XMEN disease), and protein N-glycosylation biochemistry.
| Species | Application | Title |
|---|---|---|
Nat Commun TUSC3 regulates ERMA-mediated Mg(2+) uptake for synaptic function and neurodevelopment.
| ||
Nat Commun miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC. | ||
J Allergy Clin Immunol Epigenetic activation of the TUSC3 gene as a potential therapy for XMEN disease | ||
J Transl Med Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis
| ||
Proc Natl Acad Sci U S A Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype. | ||
J Cell Biol Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
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