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Transgelin-2-specific Polyclonal antibody
Transgelin-2-specific Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15508-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IP detected in | L02 cells |
Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
15508-1-AP targets Transgelin-2-specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | transgelin 2 |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | NM_003564 |
Gene symbol | TAGLN2 |
Gene ID (NCBI) | 8407 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.
Protocols
Product Specific Protocols | |
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WB protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IHC protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IF protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IP protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
FC protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Oncol An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.
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Cancer Cell Int MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2. | ||
PLoS One Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.
| ||
Biochem Biophys Res Commun Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2. | ||
J Gastroenterol Hepatol The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma. | ||
Molecules MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway |