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- KD/KO Validated
Transgelin-2-specific Polyclonal antibody
Transgelin-2-specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15508-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, HEK-293 cells
|Positive IP detected in
|Positive IHC detected in
|human oesophagus cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15508-1-AP targets Transgelin-2-specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Rabbit / IgG
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.
|Product Specific Protocols
|WB protocol for Transgelin-2-specific antibody 15508-1-AP
|IHC protocol for Transgelin-2-specific antibody 15508-1-AP
|IF protocol for Transgelin-2-specific antibody 15508-1-AP
|IP protocol for Transgelin-2-specific antibody 15508-1-AP
|Click here to view our Standard Protocols
An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.
J Gastroenterol Hepatol
The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma.
Cancer Cell Int
MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2.
Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.
Biochem Biophys Res Commun
Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2.
MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway