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- KD/KO Validated
FAT10 Polyclonal antibody
FAT10 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (2)
WB, IHC, IF, ELISA
Cat no : 13003-2-AP
|Positive WB detected in||TNF alpha and IFN gamma treated HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 2 publications below|
|IHC||See 7 publications below|
|IF||See 3 publications below|
13003-2-AP targets FAT10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FAT10 fusion protein Ag3680|
|Full Name||ubiquitin D|
|Calculated molecular weight||165 aa, 18 kDa|
|GenBank accession number||BC012472|
|Gene ID (NCBI)||10537|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.
|Product Specific Protocols|
|WB protocol for FAT10 antibody 13003-2-AP||Download protocol|
|Click here to view our Standard Protocols|
The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.
J Biol Chem
Ubiquitin D regulates IRE1α/JNK-dependent apoptosis in pancreatic beta cells.
Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.
Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats.
c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
J Appl Toxicol
Onset of hepatocarcinogen-specific cell proliferation and cell cycle aberration during the early stage of repeated hepatocarcinogen administration in rats.