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- KD/KO Validated
FAT10 Polyclonal antibody
FAT10 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13003-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | TNF alpha and IFN gama treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
Product Information
13003-2-AP targets FAT10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAT10 fusion protein Ag3680 |
| Full Name | ubiquitin D |
| Calculated molecular weight | 165 aa, 18 kDa |
| GenBank accession number | BC012472 |
| Gene symbol | UBD |
| Gene ID (NCBI) | 10537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBD, also named as FAT10, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. UBD also has important roles in cell mitosis, chromosome instability, apoptosis and immune response. UBD mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). UBD regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. UBD may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full length UBD of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAT10 antibody 13003-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Toxicol Sci Disruption of spindle checkpoint function ahead of facilitation of cell proliferation by repeated administration of hepatocarcinogens in rats. | ||
Exp Toxicol Pathol Aberrant cell cycle regulation in rat liver cells induced by post-initiation treatment with hepatocarcinogens/hepatocarcinogenic tumor promoters. | ||
J Toxicol Sci Fluctuations in cell proliferation, apoptosis, and cell cycle regulation at the early stage of tumor promotion in rat two-stage carcinogenesis models. | ||
J Toxicol Sci Aberrant activation of ubiquitin D at G2 phase and apoptosis by carcinogens that evoke cell proliferation after 28-day administration in rats. | ||
J Appl Toxicol Onset of hepatocarcinogen-specific cell proliferation and cell cycle aberration during the early stage of repeated hepatocarcinogen administration in rats. | ||
Immunobiology c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium. |
