UBE2D1/2/3/4 Monoclonal antibody, PBS Only

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  WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68138-1-Ig (same clone as 68138-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBE2D1/2/3/4 antibody (68138-1-Ig, Clone: 1C7D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

UBE2D1/2/3/4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68138-1-PBS

Clone No.1C7D12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

UBE2D3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, E2 ubiquitin-conjugating enzyme D3, E2(17)KB3, EC:2.3.2.23

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Product Information

68138-1-PBS targets UBE2D1/2/3/4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBE2D1/2/3/4 fusion protein Ag2278
• Full Name: ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC003395
• Gene Symbol: UBE2D3
• Gene ID (NCBI): 7323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61077
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.