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  • KD/KO Validated

UHRF1 Polyclonal antibody

UHRF1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 21402-1-AP

Synonyms

hNP95, HuNp95, ICBP90, Nuclear protein 95, RING finger protein 106, RNF106, Transcription factor ICBP90, UHRF1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21402-1-AP is UHRF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UHRF1 fusion protein Ag16111
Full Name ubiquitin-like with PHD and ring finger domains 1
Calculated molecular weight 806 aa, 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC113875
Gene symbol UHRF1
Gene ID (NCBI) 29128
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.

Protocols

Product Specific Protocols
WB protocol for UHRF1 antibody 21402-1-APDownload protocol
IF protocol for UHRF1 antibody 21402-1-APDownload protocol
IP protocol for UHRF1 antibody 21402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Activated MEK/ERK Pathway Drives Widespread and Coordinated Overexpression of UHRF1 and DNMT1 in Cancer cells.

Authors - Jialun Li
humanWB

Biomed Res Int

UHRF1 Promotes Proliferation of Human Adipose-Derived Stem Cells and Suppresses Adipogenesis via Inhibiting Peroxisome Proliferator-Activated Receptor γ.

Authors - Ke Chen
  • KD Validated
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
humanWB

Int J Med Sci

Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1.

Authors - Jing Han
  • KD Validated
humanWB

Cell Physiol Biochem

Quantitative Proteomic Analysis of the Metastasis-Inhibitory Mechanism of miR-193a-3p in Non-Small Cell Lung Cancer.

Authors - Wei Deng
humanWB

Cancer Sci

Deficiency of pseudogene UPAT leads to hepatocellular carcinoma progression and forms a positive feedback loop with ZEB1.

Authors - Leyang Xiang