|Positive WB detected in||mouse skeletal muscle tissue, rat skeletal muscle tissue|
|Positive IHC detected in||human skeletal muscle tissue, human liver cancer tissue, human liver tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:300-1:1500|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20986-1-AP targets ULK1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||unc-51-like kinase 1 (C. elegans)|
|Calculated molecular weight||113 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||NM_003565|
|Gene ID (NCBI)||8408|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).
A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.
Isodunnianol alleviates doxorubicin-induced myocardial injury by activating protective autophagy.
NeuroHeal Treatment Alleviates Neuropathic Pain and Enhances Sensory Axon Regeneration.
Mol Med Rep
The antidepressant effects of apigenin are associated with the promotion of autophagy via the mTOR/AMPK/ULK1 pathway.
Int J Mol Med
CB2R orchestrates neuronal autophagy through regulation of the mTOR signaling pathway in the hippocampus of developing rats with status epilepticus.
Biochem Biophys Res Commun
Circular RNA circ_0005774 contributes to proliferation and suppresses apoptosis of acute myeloid leukemia cells via circ_0005774/miR-192-5p/ULK1 ceRNA pathway.