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ULK1 Antibody

ULK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 20986-1-AP

Synonyms

ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1



Tested Applications

Positive WB detected inrat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human liver cancer tissue, human liver tissue, mouse skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20986-1-AP targets ULK1 in IHC, WB, ELISA applications and shows reactivity with human, rat samples.

Reactivity human, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name unc-51-like kinase 1 (C. elegans)
Calculated molecular weight 113 kDa
Observed molecular weight 150 kDa
GenBank accession number NM_003565
Gene symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Protocols

Product Specific Protocols
WB protocol for ULK1 antibody 20986-1-APDownload protocol
IHC protocol for ULK1 antibody 20986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Authors - Yanan Wang
ratWB

Food Funct

Isodunnianol alleviates doxorubicin-induced myocardial injury by activating protective autophagy.

Authors - Can Chen
ratWB

Cells

NeuroHeal Treatment Alleviates Neuropathic Pain and Enhances Sensory Axon Regeneration.

Authors - David Romeo-Guitart
ratWB

Int J Mol Med

CB2R orchestrates neuronal autophagy through regulation of the mTOR signaling pathway in the hippocampus of developing rats with status epilepticus.

Authors - Qiong Wu
humanWB

Onco Targets Ther

The Acetone Indigo Red Dehydrating Agent IF203 Induces HepG2 Cell Death Through Cell Cycle Arrest, Autophagy and Apoptosis.

Authors - Yinghui Shang
ratWB

J Sex Med

Rapamycin Supplementation May Ameliorate Erectile Function in Rats With Streptozotocin-Induced Type 1 Diabetes by Inducing Autophagy and Inhibiting Apoptosis, Endothelial Dysfunction, and Corporal Fibrosis.

Authors - Huang Lin