UPF3B Polyclonal antibody

• 

  WB analysis using 23301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23301-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23301-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

UPF3B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23301-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RENT3B, hUpf3B, hUpf3p X, hUpf3p-X, MRXS14

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, K-562 cells, Jurkat cells, HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

23301-1-AP targets UPF3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPF3B fusion protein Ag18568
• Full Name: UPF3 regulator of nonsense transcripts homolog B (yeast)
• Calculated Molecular Weight: 483 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC121017
• Gene Symbol: UPF3B
• Gene ID (NCBI): 65109
• RRID: AB_2879249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZI7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of nonsense transcripts 3B (UPF3B) is also named as RENT3B, and belongs to the RENT3 family. UPF3B is a core junction protein in the Nonsense-mediated RNA decay (NMD) pathway, involved in the exonuclear transport and quality supervision of mRNA. And UPF3B showed heightened expression in hepatocellular carcinoma (HCC) tissues and was linked with adverse prognosis (PMID: 38889220). UPF3B promotes the dissociation of post-termination ribosomal complexes that lack nascent peptide. UPF3B could promote ribosome recycling, and UPF3B serves as a direct regulator of translation termination (PMID: 29333258).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Genet; Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.; Authors - Zhen Zhang