UPK3A Recombinant antibody

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  WB analysis using 83499-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83499-3-RR (UPK3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UPK3A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83499-3-RR

Clone No.240488B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83499-3-RR targets UPK3A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: uroplakin 3A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_006953
• Gene Symbol: UPK3A
• Gene ID (NCBI): 7380
• RRID: AB_3671125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75631
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.