UPK3B Polyclonal antibody

UPK3B Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15709-1-AP

Synonyms

P35, UP3b, UPIIIb, UPK3B, uroplakin 3B, Uroplakin IIIb

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Validation Data Gallery

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  IP experiment of mouse testis using 15709-1-AP

  IP result of anti-UPK3B (IP:15709-1-AP, 2ug; Detection:15709-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human bladder using 15709-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 15709-1-AP (UPK3B antibody) at dilution of 1:1000 (under 10x lens)..

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  IHC staining of human bladder using 15709-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 15709-1-AP (UPK3B antibody) at dilution of 1:1000 (under 40x lens)..

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Tested Applications

• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

The immunogen of 15709-1-AP is UPK3B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPK3B fusion protein Ag8326
• Full Name: uroplakin 3B
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC004304
• Gene Symbol: UPK3B
• Gene ID (NCBI): 80761
• RRID: AB_2878170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-3b (UPK3B), also named as p35, is a single-pass type I membrane protein. This protein dimerizes with uroplakin-1b (UPK1B) to exit out of endoplasmic reticulum, which is is an important early step in urothelial plaque assembly (PMID: 12446744).

Publications

Species	Application	Title
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
mouse	IF	Cell Rep; Cancer-associated mesothelial cells are regulated by the anti-Müllerian hormone axis; Authors - M Chauvin