Tested Applications
| Positive WB detected in | LNCaP cells, Hela cells, K-562 cells, Jurkat cells, pig brain tissue, mouse brain tissue, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67888-1-Ig targets UQCRC1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17506 Product name: Recombinant human UQCRC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 159-480 aa of BC009586 Sequence: SQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF Predict reactive species |
| Full Name | ubiquinol-cytochrome c reductase core protein I |
| Calculated Molecular Weight | 480 aa, 53 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC009586 |
| Gene Symbol | UQCRC1 |
| Gene ID (NCBI) | 7384 |
| RRID | AB_2918644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31930 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UQCRC1 antibody 67888-1-Ig | Download protocol |
| WB protocol for UQCRC1 antibody 67888-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UQCRC1 Monoclonal antibody (2G1C11), catalog number 67888-1-Ig, has a total of 2 citations. These appear in Bioorganic Chemistry and Microbiology Spectrum. The research fields covered include mitochondrial stress and apoptosis signaling in anticancer drug development, as well as PI3K/Akt/mTOR-mediated energy metabolic reprogramming and mitochondrial homeostasis during Toxoplasma gondii infection. Both studies utilized the antibody for Western blot applications in human and pig samples, respectively.
| Species | Application | Title |
|---|---|---|
Bioorg Chem SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway | ||
Microbiol Spectr PI3K/Akt/mTOR signaling pathway mediates energy metabolic reprogramming and regulates mitochondrial homeostasis in host cells exposed to Toxoplasma gondii. |
