|Positive WB detected in||Jurkat cells, HeLa cells, HepG2 cells|
|Positive IP detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
14783-1-AP targets USP36 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||USP36 fusion protein Ag6411|
|Full Name||ubiquitin specific peptidase 36|
|Calculated molecular weight||1123 aa, 123 kDa|
|Observed molecular weight||100-123 kDa|
|GenBank accession number||BC038983|
|Gene ID (NCBI)||57602|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.
Biochem Biophys Res Commun
USP36 protects proximal tubule cells from ischemic injury by stabilizing c-Myc and SOD2.
PME-1 is regulated by USP36 in ERK and Akt signaling pathways.
J Biol Chem
Loss of the Deubiquitinase USP36 Destabilizes the RNA Helicase DHX33 and Causes Preimplantation Lethality in Mice.
Nucleic Acids Res
The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.
Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.