USP36 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP36 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 14783-1-AP

Synonyms

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

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Validation Data Gallery

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  WB analysis of Jurkat using 14783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14783-1-AP (USP36 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14783-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14783-1-AP (USP36 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14783-1-AP (USP36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14783-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14783-1-AP (USP36 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14783-1-AP

  IP result of anti-USP36 (IP:14783-1-AP, 4ug; Detection:14783-1-AP 1:1000) with Jurkat cells lysate 1400 ug.

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  IF Staining of U2OS using 14783-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP36 antibody (14783-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells
• Positive IP detected in: Jurkat cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

14783-1-AP targets USP36 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP36 fusion protein Ag6411
• Full Name: ubiquitin specific peptidase 36
• Calculated Molecular Weight: 1123 aa, 123 kDa
• Observed Molecular Weight: 100-150 kDa
• GenBank Accession Number: BC038983
• Gene Symbol: USP36
• Gene ID (NCBI): 57602
• RRID: AB_2213357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nat Med; Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.; Authors - Luigina Romani
human,mouse	WB,IF	Nat Commun; USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress; Authors - Kewei Qin ; KD Validated
human	WB,IF,IP	Nucleic Acids Res; The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.; Authors - Yuanliang Yan ; KD Validated
human	WB	Cell Death Differ; Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc.; Authors - Yunhua Peng ; KO Validated
mouse	WB	Int J Mol Sci; Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity.; Authors - Marilena Pariano
human	WB, IHC, IF	Front Cell Dev Biol; USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.; Authors - Suwei Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Georgia (Verified Customer) (02-06-2023)

worked nicely in IF worked nice in WB, gave an extra band in lower molecular weight

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB. 1:1000 in TBS-5% milk, and IF 1:500
• Cell Tissue Type: HeLa