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  • KD/KO Validated

USP36 Polyclonal antibody

USP36 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14783-1-AP

Synonyms

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14783-1-AP targets USP36 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP36 fusion protein Ag6411
Full Name ubiquitin specific peptidase 36
Calculated molecular weight 1123 aa, 123 kDa
Observed molecular weight 100-123 kDa
GenBank accession numberBC038983
Gene symbol USP36
Gene ID (NCBI) 57602
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP36 antibody 14783-1-APDownload protocol
IP protocol for USP36 antibody 14783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Biochem Biophys Res Commun

USP36 protects proximal tubule cells from ischemic injury by stabilizing c-Myc and SOD2.

Authors - Qing Liu
humanWB,IP,IF

FEBS Lett

PME-1 is regulated by USP36 in ERK and Akt signaling pathways.

Authors - Soo-Yeon Kim
  • KD Validated
mouseWB

J Biol Chem

Loss of the Deubiquitinase USP36 Destabilizes the RNA Helicase DHX33 and Causes Preimplantation Lethality in Mice.

Authors - Julia M Fraile
  • KO Validated
humanWB,IF,IP

Nucleic Acids Res

The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization.

Authors - Yuanliang Yan
  • KD Validated
mouseWB

Nat Med

Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.

Authors - Luigina Romani