VAMP2 Polyclonal antibody

VAMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, Blocking, ELISA

Conjugate

Unconjugated

Cat no : 10135-1-AP

Synonyms

SYB2, Synaptobrevin 2, VAMP 2, VAMP2



Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10135-1-AP targets VAMP2 in WB, IHC, IF, Blocking, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP2 fusion protein Ag0179
Full Name vesicle-associated membrane protein 2 (synaptobrevin 2)
Calculated molecular weight 13 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC002737
Gene symbol VAMP2
Gene ID (NCBI) 6844
RRIDAB_2256918
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Protocols

Product Specific Protocols
WB protocol for VAMP2 antibody 10135-1-APDownload protocol
IHC protocol for VAMP2 antibody 10135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseBlocking

Adv Sci (Weinh)

Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.

Authors - Dongdong Wang
humanWB,IF

Adv Sci (Weinh)

Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage

Authors - Nikita Raj
IF

Neuron

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

Authors - Roberta Cagnetta
mouseIHC,IF

Brain

Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.

Authors - Lina Pan
humanIF

Sci Signal

A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity

Authors - Hyunbae Kim