VAMP2 Polyclonal antibody

VAMP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, Blocking,ELISA

Conjugate

Unconjugated

Cat no : 10135-1-AP

Synonyms

SYB2, Synaptobrevin 2, VAMP 2, VAMP2



Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10135-1-AP targets VAMP2 in WB, IHC, IF, Blocking,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP2 fusion protein Ag0179
Full Name vesicle-associated membrane protein 2 (synaptobrevin 2)
Calculated molecular weight 13 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC002737
Gene symbol VAMP2
Gene ID (NCBI) 6844
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Protocols

Product Specific Protocols
WB protocol for VAMP2 antibody 10135-1-APDownload protocol
IHC protocol for VAMP2 antibody 10135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
humanWB

J Biol Chem

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Authors - Chengfeng Merriman
mouseWB

J Biol Chem

Loss of the voltage-gated proton channel Hv1 decreases insulin secretion and leads to hyperglycemia and glucose intolerance in mice.

Authors - Huimin Pang
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Authors - Sara Sancho-Knapik
humanWB

J Biol Chem

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

Authors - Wulin Aerbajinai
mouseWB

Clin Sci (Lond)

Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.

Authors - Jizheng Chen