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VAMP3/Cellubrevin Polyclonal antibody
VAMP3/Cellubrevin Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, Blocking, ELISA
Cat no : 10702-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, MCF-7 cells, mouse brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 10 publications below|
|IHC||See 1 publications below|
|IF||See 6 publications below|
10702-1-AP targets VAMP3/Cellubrevin in WB, IP, IHC, IF, Blocking, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAMP3/Cellubrevin fusion protein Ag1156|
|Full Name||vesicle-associated membrane protein 3 (cellubrevin)|
|Calculated molecular weight||11 kDa|
|Observed molecular weight||11-17 kDa|
|GenBank accession number||BC005941|
|Gene ID (NCBI)||9341|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.
|Product Specific Protocols|
|WB protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|IHC protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|IP protocol for VAMP3/Cellubrevin antibody 10702-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Adv Sci (Weinh)
Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage
Adv Sci (Weinh)
Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.
KRAS regulation by small non-coding RNAs and SNARE proteins.
A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.
Front Cell Dev Biol
VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.
Multifunctional regulation of VAMP3 in exocytic and endocytic pathways of RBL-2H3 cells