VAMP3/Cellubrevin Polyclonal antibody

• Featured Product
• KD/KO Validated

VAMP3/Cellubrevin Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, Blocking, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 10702-1-AP

Synonyms

CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 10702-1-AP

  WB result of VAMP3/Cellubrevin antibody (10702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAMP3/Cellubrevin transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 10702-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10702-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10702-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10702-1-AP

  IP Result of anti-VAMP3/Cellubrevin (IP:10702-1-AP, 4ug; Detection:10702-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

• 

  IHC staining of human heart using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

10702-1-AP targets VAMP3/Cellubrevin in WB, IP, IHC, IF, Blocking, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAMP3/Cellubrevin fusion protein Ag1156
• Full Name: vesicle-associated membrane protein 3 (cellubrevin)
• Calculated molecular weight: 11 kDa
• Observed molecular weight: 11-17 kDa
• GenBank accession number: BC005941
• Gene symbol: VAMP3
• Gene ID (NCBI): 9341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Publications

Species	Application	Title
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human	IHC	Oncotarget; A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.; Authors - Mitsuhiro Shimura
mouse	IF	Front Cell Dev Biol; VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.; Authors - Juan-Juan Zhu
rat	WB	Front Immunol; Multifunctional regulation of VAMP3 in exocytic and endocytic pathways of RBL-2H3 cells; Authors - Satomi Mishima

Related Products

VAMP1 Polyclonal antibody

13115-1-AP

VAMP2 Polyclonal antibody

10135-1-AP

BVES Polyclonal antibody

12920-1-AP

BAP31 Polyclonal antibody

11200-1-AP

SNAP23 Polyclonal antibody

10825-1-AP

VAMP7/TI-VAMP Polyclonal antibody

22268-1-AP

VAMP8 Polyclonal antibody

15546-1-AP

Syntaxin 6 Polyclonal antibody

10841-1-AP

Syntaxin 6 Monoclonal antibody

60059-1-Ig