VAMP7/TI-VAMP Polyclonal antibody

VAMP7/TI-VAMP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, Blocking,ELISA

Conjugate

Unconjugated

Cat no : 22268-1-AP

Synonyms

SYBL1, Synaptobrevin like protein 1, Tetanus insensitive VAMP, TI VAMP, TI-VAMP, VAMP 7, VAMP7



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

22268-1-AP targets VAMP7/TI-VAMP in WB, IP, IHC, IF, Blocking,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name vesicle-associated membrane protein 7
Calculated molecular weight 25 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberNM_005638
Gene symbol VAMP7
Gene ID (NCBI) 6845
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP7, also named as SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is required for calcium regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.

Protocols

Product Specific Protocols
WB protocol for VAMP7/TI-VAMP antibody 22268-1-APDownload protocol
IHC protocol for VAMP7/TI-VAMP antibody 22268-1-APDownload protocol
IF protocol for VAMP7/TI-VAMP antibody 22268-1-APDownload protocol
IP protocol for VAMP7/TI-VAMP antibody 22268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Small

Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.

Authors - Jia Liu
mouseBlocking

Adv Sci (Weinh)

Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.

Authors - Dongdong Wang