- Featured Product
- KD/KO Validated
VAMP7/TI-VAMP Polyclonal antibody
VAMP7/TI-VAMP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 22268-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, rat brain tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|human liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, HeLa cells
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22268-1-AP targets VAMP7/TI-VAMP in WB, IP, IHC, IF, Blocking, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|vesicle-associated membrane protein 7
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
VAMP7, also named as SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is required for calcium regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.
|Product Specific Protocols
|WB protocol for VAMP7/TI-VAMP antibody 22268-1-AP
|IHC protocol for VAMP7/TI-VAMP antibody 22268-1-AP
|IF protocol for VAMP7/TI-VAMP antibody 22268-1-AP
|IP protocol for VAMP7/TI-VAMP antibody 22268-1-AP
|Click here to view our Standard Protocols
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