VAMP8 Polyclonal antibody

VAMP8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15546-1-AP

Synonyms

EDB, Endobrevin, VAMP 8, VAMP8

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Validation Data Gallery

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  WB analysis using 15546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15546-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15546-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-12 cells, THP-1 cells
• Positive IHC detected in: human placenta tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

15546-1-AP targets VAMP8 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAMP8 fusion protein Ag7903
• Full Name: vesicle-associated membrane protein 8 (endobrevin)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001634
• Gene Symbol: VAMP8
• Gene ID (NCBI): 8673
• RRID: AB_2878150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

Publications

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
rat	WB	Arch Toxicol; Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion.; Authors - Huaisha Xu
human	WB	Ecotoxicol Environ Saf; Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.; Authors - Yuanli Zhang
rat	WB	Stem Cell Res Ther; Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells.; Authors - Zheng Li
human	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin