VAMP8 Polyclonal antibody

VAMP8 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 15546-1-AP

Synonyms

EDB, Endobrevin, VAMP 8, VAMP8



Tested Applications

Positive WB detected inHL-60 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

15546-1-AP targets VAMP8 in WB, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP8 fusion protein Ag7903
Full Name vesicle-associated membrane protein 8 (endobrevin)
Calculated molecular weight 11 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC001634
Gene symbol VAMP8
Gene ID (NCBI) 8673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

Protocols

Product Specific Protocols
WB protocol for VAMP8 antibody 15546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Autophagy impairment as a key feature for acetaminophen-induced ototoxicity.

Authors - Tong Zhao
mouseWB

Cell Death Dis

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Authors - Yan Cao
ratWB, CoIP

Biochem Biophys Res Commun

Mild hypothermia pretreatment extenuates liver ischemia-reperfusion injury through Rab7-mediated autophagosomes-lysosomes fusion.

Authors - Anxiong Liu
ratWB

Stem Cell Res Ther

Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells.

Authors - Zheng Li