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  • KD/KO Validated

VCAM-1 Antibody

VCAM-1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11444-1-AP

Synonyms

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human spleen tissue, human placenta tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

11444-1-AP targets VCAM-1 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VCAM-1 fusion protein Ag1993
Full Name vascular cell adhesion molecule 1
Calculated molecular weight 739aa,81 kDa
Observed molecular weight
GenBank accession number BC017276
Gene symbol VCAM1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
IHC protocol for VCAM-1 antibody 11444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,ratWB

FASEB J

Fra-1 plays a critical role in angiotensin II-induced vascular senescence.

Authors - Di Yang
humanIF

Cell Death Dis

Cancer-associated fibroblasts enhance tumor-associated macrophages enrichment and suppress NK cells function in colorectal cancer.

Authors - Rongsheng Zhang
  • KD Validated
humanWB

J Cardiovasc Pharmacol Ther

Chrysin Suppresses Vascular Endothelial Inflammation via Inhibiting the NF-κB Signaling Pathway.

Authors - Shengnan Zhao
mouseIF

Biochem Biophys Res Commun

Cyclophilin D deficiency protects against the development of mitochondrial ROS and cellular inflammation in aorta.

Authors - Xiaojing Liu
mouse,ratWB,IF

Cardiovasc Res

HDAC4 regulates vascular inflammation via activation of autophagy.

Authors - Di Yang
humanWB

Acta Biochim Biophys Sin (Shanghai)

SET8 is involved in the regulation of hyperglycemic memory in human umbilical endothelial cells.

Authors - Xiangyuan Chen