|Positive WB detected in||human spleen tissue|
|Positive IHC detected in||human tonsillitis tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66294-1-Ig targets VCAM-1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||VCAM-1 fusion protein Ag24697|
|Full Name||vascular cell adhesion molecule 1|
|Calculated molecular weight||739 aa, 81 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC017276|
|Gene ID (NCBI)||7412|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).
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