VCAM-1 Monoclonal antibody

VCAM-1 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B12F12

Cat no : 66294-1-Ig

Synonyms

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66294-1-Ig targets VCAM-1 in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VCAM-1 fusion protein Ag24697
Full Name vascular cell adhesion molecule 1
Calculated molecular weight 739 aa, 81 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC017276
Gene symbol VCAM1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
IHC protocol for VCAM-1 antibody 66294-1-IgDownload protocol
IF protocol for VCAM-1 antibody 66294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Redox Biol

Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.

Authors - Zhuqing Li
humanWB

J Hazard Mater

Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells.

Authors - Xuting Liu
mouseWB

J Nanobiotechnology

Macrophage-cancer hybrid membrane-coated nanoparticles for targeting lung metastasis in breast cancer therapy.

Authors - Chunai Gong
humanWB

J Mol Cell Cardiol

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Authors - Xinxing Zhu
humanWB

J Cell Mol Med

Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation

Authors - Peng Liu
humanWB

Phytomedicine

Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3.

Authors - Tong Lin