VCAM-1 Monoclonal antibody

VCAM-1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1B12F12

Cat no : 66294-1-Ig

Synonyms

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66294-1-Ig targets VCAM-1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VCAM-1 fusion protein Ag24697
Full Name vascular cell adhesion molecule 1
Calculated molecular weight 739 aa, 81 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC017276
Gene symbol VCAM1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
WB protocol for VCAM-1 antibody 66294-1-IgDownload protocol
IHC protocol for VCAM-1 antibody 66294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

PMID: 29452166

Authors - Qiangbing Yang
humanWB

J Recept Signal Transduct Res

Chicoric acid attenuates hyperglycemia-induced endothelial dysfunction through AMPK-dependent inhibition of oxidative/nitrative stresses.

Authors - Xiaojuan Ma
mouseWB

Cell J

Protection against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Neferine, A Natural Product from Nelumbo nucifera Gaertn.

Authors - Xiangjing Min
humanWB

Acta Biochim Biophys Sin (Shanghai)

PMID: 31650167

Authors - Jie Qi
humanWB

J Appl Toxicol

The role of AMP-activated protein kinase α1-mediated endoplasmic reticulum stress in alleviating the toxic effect of uremic toxin indoxyl sulfate on vascular endothelial cells by Klotho.

Authors - Cheng Chen
humanWB

Biol Res

CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis.

Authors - Xiaohao Zhang