VCAM-1 Monoclonal antibody

VCAM-1 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human and More (1)







Cat no : 66294-1-Ig


CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66294-1-Ig targets VCAM-1 in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VCAM-1 fusion protein Ag24697
Full Name vascular cell adhesion molecule 1
Calculated Molecular Weight 739 aa, 81 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC017276
Gene Symbol VCAM1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).


Product Specific Protocols
IHC protocol for VCAM-1 antibody 66294-1-IgDownload protocol
IF protocol for VCAM-1 antibody 66294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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