VCAN Polyclonal antibody, PBS Only

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  WB analysis using 30599-1-AP (same clone as 30599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30599-1-AP (VCAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

VCAN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 30599-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CSPG2, ERVR, GHAP, Glial hyaluronate-binding protein, Large fibroblast proteoglycan

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Product Information

30599-1-PBS targets VCAN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VCAN fusion protein Ag30151
• Full Name: versican
• Calculated Molecular Weight: 373 kDa
• Observed Molecular Weight: 250-300 kDa, 550 kDa
• GenBank Accession Number: NM_004385
• Gene Symbol: VCAN
• Gene ID (NCBI): 1462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13611
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).