VCP Polyclonal antibody

• 

  WB analysis of U2OS using 10736-1-AP

  U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 10736-1-AP incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10736-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10736-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 10736-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.

• 

  IP experiment of mouse brain using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:300) with mouse brain tissue lysate 2400 ug.

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

VCP Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10736-1-AP

Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

---

Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, rat lung tissue, mouse lung tissue, U2OS cells, C6 cells, mouse thymus tissue, mouse brain tissue, rat brain tissue, SH-SY5Y cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 49 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

10736-1-AP targets VCP in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1002

  Product name: Recombinant human VCP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 345-644 aa of BC007562

  Sequence: GMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG

• Full Name: valosin-containing protein
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC007562
• Gene Symbol: VCP
• Gene ID (NCBI): 7415
• RRID: AB_2214635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55072
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Nat Commun; Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.; Authors - Rommel J Gestuveo ; KD Validated
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang