Validation Data Gallery
|Positive WB detected in||HeLa cells, human brain tissue, rat lung tissue, mouse lung tissue, mouse thymus tissue, C6 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
10736-1-AP targets VCP in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VCP fusion protein Ag1002|
|Full Name||valosin-containing protein|
|Calculated molecular weight||89 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC007562|
|Gene ID (NCBI)||7415|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus.
Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.
ACS Chem Neurosci
Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.
Cell Death Dis
SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells.
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
J Exp Clin Cancer Res
MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.