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  • KD/KO Validated

VCP Polyclonal antibody

VCP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10736-1-AP

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP



Tested Applications

Positive WB detected inC6 cells, rat lung tissue, mouse lung tissue, mouse brain tissue, RAW264.7, rat brain tissue, SH-SY5Y cells, human brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10736-1-AP targets VCP in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VCP fusion protein Ag1002
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC007562
Gene symbol VCP
Gene ID (NCBI) 7415
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
WB protocol for VCP antibody 10736-1-APDownload protocol
IHC protocol for VCP antibody 10736-1-APDownload protocol
IF protocol for VCP antibody 10736-1-APDownload protocol
IP protocol for VCP antibody 10736-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.

Authors - Sebastian Markmiller
mouseWB

ACS Chem Neurosci

Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.

Authors - Yiming Pan
humanWB

Cell Death Dis

SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells.

Authors - Juri Na
humanWB

Cell Rep

p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.

Authors - Susan Kilgas
  • KD Validated
humanWB

J Exp Clin Cancer Res

MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.

Authors - Yang Wang
  • KD Validated
ratWB

Acupunct Med

Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.

Authors - Yang Zhang