|Positive WB detected in||C6 cells, rat lung tissue, mouse lung tissue, mouse brain tissue, RAW264.7, rat brain tissue, SH-SY5Y cells, human brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10736-1-AP targets VCP in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VCP fusion protein Ag1002|
|Full Name||valosin-containing protein|
|Calculated molecular weight||89 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC007562|
|Gene ID (NCBI)||7415|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
|Product Specific Protocols|
|WB protocol for VCP antibody 10736-1-AP||Download protocol|
|IHC protocol for VCP antibody 10736-1-AP||Download protocol|
|IF protocol for VCP antibody 10736-1-AP||Download protocol|
|IP protocol for VCP antibody 10736-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.
ACS Chem Neurosci
Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.
Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse.
Mol Cell Biol
The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.
Mol Cell Proteomics
Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers.
Hum Mol Genet
Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.