|Positive WB detected in||C6 cells, rat lung tissue, mouse lung tissue, mouse brain tissue, RAW264.7, rat brain tissue, SH-SY5Y cells, human brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10736-1-AP targets VCP in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VCP fusion protein Ag1002|
|Full Name||valosin-containing protein|
|Calculated molecular weight||89 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC007562|
|Gene ID (NCBI)||7415|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
|Product Specific Protocols|
|WB protocol for VCP antibody 10736-1-AP||Download protocol|
|IHC protocol for VCP antibody 10736-1-AP||Download protocol|
|IF protocol for VCP antibody 10736-1-AP||Download protocol|
|IP protocol for VCP antibody 10736-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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