VEGFA Polyclonal antibody
VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, FC, CoIP, ELISA
Cat no : 26381-1-AP
|Positive WB detected in||HEK-293T cells, HeLa cells, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 4 publications below|
|IHC||See 2 publications below|
|CoIP||See 1 publications below|
26381-1-AP targets VEGFA in WB, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||VEGFA fusion protein Ag24740|
|Full Name||vascular endothelial growth factor A|
|Calculated molecular weight||412 aa, 46 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC065522|
|Gene ID (NCBI)||7422|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).
|Product Specific Protocols|
|WB protocol for VEGFA antibody 26381-1-AP||Download protocol|
|IHC protocol for VEGFA antibody 26381-1-AP||Download protocol|
|FC protocol for VEGFA antibody 26381-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury
J Cell Mol Med
Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.
Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.
Cell Death Discov
Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.