VEGFA Polyclonal antibody

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  WB analysis using 26381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26381-1-AP (VEGFA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 40x lens).

VEGFA Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26381-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, CoIP, ELISA

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

26381-1-AP targets VEGFA in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag24740
• Full Name: vascular endothelial growth factor A
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC065522
• Gene Symbol: VEGFA
• Gene ID (NCBI): 7422
• RRID: AB_2880496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

Publications

Species	Application	Title
human	WB	Antioxidants (Basel); Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury; Authors - Corry D Bondi
human	WB,CoIP, IHC	J Cell Mol Med; Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.; Authors - Xiaofei Wang
mouse,human	WB	J Stroke Cerebrovasc Dis; LncRNA SNHG12 regulated by WTAP aggravated the oxygen-glucose deprivation/reperfusion-induced injury in bEnd.3 cell; Authors - Faqing Long
human	WB,IHC	Oncol Lett; Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.; Authors - Yu Miao
human	WB	Cell Death Discov; Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.; Authors - Longyang Jin
human	WB	Discov Oncol; CCL28 promotes progression of hepatocellular carcinoma through PDGFD-regulated MMP9 and VEGFA pathways; Authors - Youyi Liu