VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26381-1-AP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26381-1-AP targets VEGFA in WB, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFA fusion protein Ag24740
Full Name vascular endothelial growth factor A
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC065522
Gene symbol VEGFA
Gene ID (NCBI) 7422
RRIDAB_2880496
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

Protocols

Product Specific Protocols
WB protocol for VEGFA antibody 26381-1-APDownload protocol
IHC protocol for VEGFA antibody 26381-1-APDownload protocol
FC protocol for VEGFA antibody 26381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Antioxidants (Basel)

Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury

Authors - Corry D Bondi
humanWB,CoIP, IHC

J Cell Mol Med

Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.

Authors - Xiaofei Wang
humanWB,IHC

Oncol Lett

Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.

Authors - Yu Miao
humanWB

Cell Death Discov

Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.

Authors - Longyang Jin