VEGFA Polyclonal antibody
VEGFA Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 26381-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 26381-1-AP is VEGFA Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VEGFA fusion protein Ag24740 |
Full Name | vascular endothelial growth factor A |
Calculated molecular weight | 412 aa, 46 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC065522 |
Gene symbol | VEGFA |
Gene ID (NCBI) | 7422 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for VEGFA antibody 26381-1-AP | Download protocol |
IHC protocol for VEGFA antibody 26381-1-AP | Download protocol |
FC protocol for VEGFA antibody 26381-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Discov Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer. | ||
Oncol Lett Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer. | ||
J Cell Mol Med Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis. |