VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26381-1-AP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Validation Data Gallery

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  WB analysis using 26381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26381-1-AP (VEGFA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of NIH/3T3 using 26381-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human VEGFA (26381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 26381-1-AP is VEGFA Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag24740
• Full Name: vascular endothelial growth factor A
• Calculated molecular weight: 412 aa, 46 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Cell Death Discov; Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.; Authors - Longyang Jin
human	WB,IHC	Oncol Lett; Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.; Authors - Yu Miao
human	WB,CoIP, IHC	J Cell Mol Med; Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.; Authors - Xiaofei Wang