VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26381-1-AP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Validation Data Gallery

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  WB analysis using 26381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26381-1-AP (VEGFA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26381-1-AP is VEGFA Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag24740
• Full Name: vascular endothelial growth factor A
• Calculated molecular weight: 412 aa, 46 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.