VGLUT1 Polyclonal antibody

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  WB analysis of mouse brain using 29469-1-AP

  Various tissue were subjected to SDS PAGE followed by western blot with 29469-1-AP (VGLUT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

VGLUT1 Polyclonal Antibody for WB, ELISA

Cat No. 29469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SLC17A7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, BNPI

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Tested Applications

• Positive WB detected in: unboiled mouse brain tissue, unboiled rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29469-1-AP targets VGLUT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VGLUT1 fusion protein Ag31193
• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_020309
• Gene Symbol: VGLUT1
• Gene ID (NCBI): 57030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2U7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons. VGLUT1 has several isoforms with the moleculat weight range from 53-61 kDa.

VGLUT1 is a multiple transmembrane protein, which is easily to aggregate if the sample is bolied or treated with high temperature, thus we recommend to use unboiled sample for the detection.