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VMAT2 Polyclonal antibody

VMAT2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20873-1-AP

Synonyms

Monoamine transporter, SLC18A2, SVAT, SVMT, VAT2, Vesicular amine transporter 2, VMAT2



Tested Applications

Positive WB detected inSGC-7901 cells, Jurkat cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20873-1-AP targets VMAT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VMAT2 fusion protein Ag14971
Full Name solute carrier family 18 (vesicular monoamine), member 2
Calculated Molecular Weight 514 aa, 56 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC108928
Gene Symbol SLC18A2
Gene ID (NCBI) 6571
RRIDAB_10858619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VMAT2 is a vesicular monoamine transporter that is responsible for the uptake and storage of monoamines (dopaine, norepinephrine, epinephrine, histamine and serotonin) in neurons, endocrine cells, and tumors deriving from these cells. Histamine-producing endocrine tumors (gastric carcinoids) expressed VMAT2 almost exclusively, thus VMAT2 is useful in classification of neuroendocrine tumors. Reduced expression of VMAT2 has often been observed in Parkinson's diseased (PD) brain, and detection of VMAT2 can be used to reflect the 4-(2-aminoethyl)benzene-1,2-diol neuron loss and predict the disease process. Several forms of VMAT2 can be observed upon the glycosylation: 45 kDa band corresponds to the native (deglycosylated) form, and the 55 and 75 kDa bands to glycosylated forms (PMID: 17582657).

Protocols

Product Specific Protocols
WB protocol for VMAT2 antibody 20873-1-APDownload protocol
IHC protocol for VMAT2 antibody 20873-1-APDownload protocol
IF protocol for VMAT2 antibody 20873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
humanIF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
humanWB

Dis Model Mech

A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.

Authors - Zhao-Feng Li
humanIF

Front Neurosci

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease

Authors - Fahad Mubariz
ratWB

Parkinsons Dis

PMID: 30662707

Authors - Haruo Nishijima
humanIF

Mol Med

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Authors - Sha Li