VMAT2 Polyclonal antibody

VMAT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20873-1-AP

Synonyms

Monoamine transporter, SLC18A2, SVAT, SVMT, VAT2, Vesicular amine transporter 2, VMAT2



Tested Applications

Positive WB detected inSGC-7901 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20873-1-AP is VMAT2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VMAT2 fusion protein Ag14971
Full Name solute carrier family 18 (vesicular monoamine), member 2
Calculated molecular weight 514 aa, 56 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC108928
Gene symbol SLC18A2
Gene ID (NCBI) 6571
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VMAT2 is a vesicular monoamine transporter that is responsible for the uptake and storage of monoamines (dopaine, norepinephrine, epinephrine, histamine and serotonin) in neurons, endocrine cells, and tumors deriving from these cells. Histamine-producing endocrine tumors (gastric carcinoids) expressed VMAT2 almost exclusively, thus VMAT2 is useful in classification of neuroendocrine tumors. Reduced expression of VMAT2 has often been observed in Parkinson’s diseased (PD) brain, and detection of VMAT2 can be used to reflect the 4-(2-aminoethyl)benzene-1,2-diol neuron loss and predict the disease process. VMAT2 migrated as a band of 65-75 kDa and a lower band of 55-60 kDa, representing the glycosylated form and native form.

Protocols

Product Specific Protocols
WB protocol for VMAT2 antibody 20873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Parkinsons Dis

PMID: 30662707

Authors - Haruo Nishijima
humanIF

Mol Med

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Authors - Sha Li
humanIF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
mouseWB,IF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian