|Positive WB detected in||SGC-7901 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20873-1-AP is VMAT2 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VMAT2 fusion protein Ag14971|
|Full Name||solute carrier family 18 (vesicular monoamine), member 2|
|Calculated molecular weight||514 aa, 56 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC108928|
|Gene ID (NCBI)||6571|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VMAT2 is a vesicular monoamine transporter that is responsible for the uptake and storage of monoamines (dopaine, norepinephrine, epinephrine, histamine and serotonin) in neurons, endocrine cells, and tumors deriving from these cells. Histamine-producing endocrine tumors (gastric carcinoids) expressed VMAT2 almost exclusively, thus VMAT2 is useful in classification of neuroendocrine tumors. Reduced expression of VMAT2 has often been observed in Parkinson’s diseased (PD) brain, and detection of VMAT2 can be used to reflect the 4-(2-aminoethyl)benzene-1,2-diol neuron loss and predict the disease process. VMAT2 migrated as a band of 65-75 kDa and a lower band of 55-60 kDa, representing the glycosylated form and native form.
Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.
Identification of TMEM230 mutations in familial Parkinson's disease.
Reversing a model of Parkinson's disease with in situ converted nigral neurons.