VPS33B Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS33B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 12195-1-AP

Synonyms

Vacuolar protein sorting-associated protein 33B, hVPS33B

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 12195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12195-1-AP

  WB result of VPS33B antibody (12195-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS33B transfected HEK-293 cells.

• 

  WB analysis of HeLa using 12195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12195-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12195-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12195-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12195-1-AP

  Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody 12195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue, HeLa cells, mouse testis tissue, L02 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12195-1-AP targets VPS33B in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS33B fusion protein Ag2833
• Full Name: vacuolar protein sorting 33 homolog B (yeast)
• Calculated Molecular Weight: 617 aa, 71 kDa
• Observed Molecular Weight: 65-71 kDa
• GenBank Accession Number: BC016445
• Gene Symbol: VPS33B
• Gene ID (NCBI): 26276
• RRID: AB_2215198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Nat Genet; Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.; Authors - Cullinane Andrew R AR ; KD Validated
mouse	WB	Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
human	WB,IF	Proc Natl Acad Sci U S A; Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.; Authors - Wong Dennis D
human	IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R
human	WB	Clin Kidney J; Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.; Authors - Amelia Holme
human	IHC, WB, CoIP, ChIP	Cancer Sci; VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.; Authors - Yingxia Ning

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (11-03-2020)

Excellent antibody for wester and IF.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

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