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  • KD/KO Validated

VPS33B Polyclonal antibody

VPS33B Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12195-1-AP



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, HeLa cells, mouse testis tissue, L02 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12195-1-AP targets VPS33B in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33B fusion protein Ag2833
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated Molecular Weight 617 aa, 71 kDa
Observed Molecular Weight 65-71 kDa
GenBank Accession NumberBC016445
Gene Symbol VPS33B
Gene ID (NCBI) 26276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).


Product Specific Protocols
WB protocol for VPS33B antibody 12195-1-APDownload protocol
IHC protocol for VPS33B antibody 12195-1-APDownload protocol
IF protocol for VPS33B antibody 12195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Genet

Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.

Authors - Cullinane Andrew R AR
  • KD Validated

Nat Cell Biol

Circadian control of the secretory pathway maintains collagen homeostasis.

Authors - Joan Chang

Proc Natl Acad Sci U S A

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Authors - Wong Dennis D

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R

Clin Kidney J

Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.

Authors - Amelia Holme
humanIHC, WB, CoIP, ChIP

Cancer Sci

VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.

Authors - Yingxia Ning


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


RICHA (Verified Customer) (11-03-2020)

Excellent antibody for wester and IF.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast