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VPS33B Polyclonal antibody
VPS33B Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 12195-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse skeletal muscle tissue, HeLa cells, mouse testis tissue, L02 cells|
|Positive IHC detected in||human colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12195-1-AP targets VPS33B in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPS33B fusion protein Ag2833|
|Full Name||vacuolar protein sorting 33 homolog B (yeast)|
|Calculated molecular weight||617 aa, 71 kDa|
|Observed molecular weight||65-71 kDa|
|GenBank accession number||BC016445|
|Gene ID (NCBI)||26276|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).
Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.
Nat Cell Biol
Circadian control of the secretory pathway maintains collagen homeostasis.
Proc Natl Acad Sci U S A
Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.
J Cell Sci
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
Clin Kidney J
Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.
VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
RICHA (Verified Customer) (11-03-2020)
Excellent antibody for wester and IF.