VPS35 Polyclonal antibody

VPS35 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10236-1-AP

Synonyms

hVPS35, Maternal embryonic 3, MEM3, Vesicle protein sorting 35, VPS35



Tested Applications

Positive WB detected inA549 cells, mouse kidney tissue, mouse brain tissue, rat liver tissue, HepG2 cells, rat kidney tissue, HEK-293 cells
Positive IP detected inmouse kidney tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10236-1-AP targets VPS35 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS35 fusion protein Ag0340
Full Name vacuolar protein sorting 35 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC002414
Gene symbol VPS35
Gene ID (NCBI) 55737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS35 protein belongs to a group of vacuolar protein sorting (VPS) proteins, which ensure the proper delivery of organelle-specific proteins in eukaryotic cells. VPS35 is the core of a multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Vps35 serves as the core of the multimeric complex by binding directly to Vps26 and Vps29 and SNX1. Northern blot analyses in 16 tissues showed that one transcript of Vps35 with a size of 3.6 kb was highly expressed in brain, heart, testis, ovary, small intestine, spleen, skeletal muscle, and placenta and expressed at moderate or low levels in other tissues. Another transcript of Vps35, a message of 3.0 kb, was also expressed with proportionally lower levels than the 3.6-kb transcript in all the tissues except that the 3.0-kb transcript was not detected in brain. Human Vps35 is mapped at 16q13-q21.

Protocols

Product Specific Protocols
WB protocol for VPS35 antibody 10236-1-APDownload protocol
IF protocol for VPS35 antibody 10236-1-APDownload protocol
IP protocol for VPS35 antibody 10236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Authors - Xuan Ling Hilary Yong
ratWB,IHC

Neural Regen Res

Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35.

Authors - Mingmin Yan
mouseWB

J Neurochem

Retromer and Rab2-dependent trafficking mediate PS1 degradation by proteasomes in endocytic disturbance.

Authors - Naoya Ueda
  • KD Validated
monkeyWB,IF

Am J Pathol

Dynein Dysfunction Reproduces Age-Dependent Retromer Deficiency: Concomitant Disruption of Retrograde Trafficking Is Required for Alteration in β-Amyloid Precursor Protein Metabolism.

Authors - Nobuyuki Kimura
WB

Hum Mol Genet

VPS35 pathogenic mutations confer no dominant toxicity but partial loss of function in Drosophila and genetically interact with parkin.

Authors - Bilal R Malik
ratWB,CoIP,IF

Cell Death Differ

Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.

Authors - Jing Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

stephen (Verified Customer) (09-07-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours vps35 antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: mouse OC2 cells
VPS35 Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC2 cells (Cat no:10236-1-AP)