VWF, VWFpp Polyclonal antibody

VWF, VWFpp Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 11778-1-AP

Synonyms

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF, VWF, VWFpp



Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

11778-1-AP targets VWF, VWFpp in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VWF, VWFpp fusion protein Ag2386
Full Name von Willebrand factor
Calculated molecular weight 309 kDa
Observed molecular weight 309-320 kDa, 75-100 kDa
GenBank accession number.
Gene symbol VWF
Gene ID (NCBI) 7450
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Von Willebrand factor (vWF) is a large multimeric glycoprotein that is crucial to the hemostasis process. It supports platelet adhesion and carries factor VIII (FVIII). Deficiency of vWF results in von Willebrand disease (vWD), a common inherited bleeding disorder. The pre-pro-vWF is comprised of 2,813 amino acids (aa) which encompass a 22-aa signal peptide, a 741-aa large propeptide (vWF propeptide, vWFpp, also called von Willebrand antigen 2) and 2,050 aa making up the mature vWF. The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. This antibody raised against the N-terminal region of pre-pro-vWF recognizes von Willebrand antigen 2 (75-100 kDa) and pro-vWF (309-320 kDa). (PMID: 9759493; 12067899; 22452980; 8874191)

Protocols

Product Specific Protocols
WB protocol for VWF, VWFpp antibody 11778-1-APDownload protocol
IP protocol for VWF, VWFpp antibody 11778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Evid Based Complement Alternat Med

Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways.

Authors - Rong Yuan
humanIF

J Mol Cell Cardiol

Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates endothelial cell activation.

Authors - Barbara Kutryb-Zajac
mouseIHC

Vet Pathol

A Transplantable Syngeneic Allograft Mouse Model for Nongestational Choriocarcinoma of the Ovary.

Authors - Ludmila Szabova
humanWB

Theranostics

Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer.

Authors - Zitong Zhao
ratIF

Neuroscience

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats.

Authors - Liu Liu
humanIF

Theranostics

Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction.

Authors - Yu Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kim-Anh (Verified Customer) (09-03-2019)

Good primary antibody, I used for IF test on HUVECs

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: huvecs