Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)
EthD-I (DNA binded): Ex/Em
Cat no : PF00008
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems.
This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
A.Calcein AM (4 mM in anhydrous DMSO)
B.Ethidium homodimer-1 (EthD-I) (2 mM in DMSO/H2O 1:4 (v/v))
Note: The test size is determined as 0.5 mL working solution for one sample in flow cytometry.
Store at -20°C. Avoid exposure to light. Stable for 6 months after shipment.
Note that Calcein M is easy to hydrolyze, so it needs to be sealed and stored dry, and the diluted working solution needs to be prepared the same day.
1. Fluorescent dyes all have quenching problems. Please try to avoid light during storage and use to slow down fluorescence quenching.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
Calcein AM：Ex/Em = 494/517 nm
EthD-I: Ex/Em = 528/617 nm (binding DNA)