• Featured Product
  • KD/KO Validated

Vimentin Recombinant antibody

Vimentin Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

6K21

Cat no : 80232-1-RR

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin



Tested Applications

Positive WB detected inMCF-7 cells, U-251 cells, A549 cells, HeLa cells, U2OS cells, NIH/3T3 cells, 4T1 cells, ROS1728 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1250-1:5000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

80232-1-RR targets Vimentin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 80232-1-RRDownload protocol
IHC protocol for Vimentin antibody 80232-1-RRDownload protocol
IF protocol for Vimentin antibody 80232-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols