ChromoTek Vimentin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Vimentin

Cat no : vcg

Synonyms



Product Information

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

Applications Live cell imaging
SpecificityVimentin intermediate filament protein, tested in human, hamster & dog cells
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Vimentin single domain antibody (sdAb) fragment
SequenceWith the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Methods Cell Biol

Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.

Authors - Catherine S Schrankel
IF

Cancer Res

Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.

Authors - Julia Maier