ChromoTek Vimentin-Chromobody® plasmid (TagGFP)
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagGFP2
Epitope
Vimentin
Cat no : vcg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.
| Applications | Live cell imaging |
| Specificity | Vimentin intermediate filament protein, tested in human, hamster & dog cells |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Reporter | TagGFP2 |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Encoded Nanobody/ VHH | Monoclonal anti-Vimentin single domain antibody (sdAb) fragment |
| Sequence | With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Storage | Shipped at ambient temperature. Store at -20 °C |
Publications
| Application | Title |
|---|---|
Methods Cell Biol Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos. | |
Cancer Res Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology. |