Cat no : vcg
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.
|Description||The Vimentin-Chromobody visualizes endogenous Vimentin in real time.
• Easily transfect your cells with the Vimentin-Chromobody plasmid
• Use Vimentin-Chromobody as a marker of vimentin cytoskeleton
• Monitor dynamics of vimentin intermediate filaments in live cells
• Vimentin-Chromobody does not affect cell viability
• The binding of Vimentin-Chromobody does not influence cell viability or cell motility and thus offers you the unique possibility to non-invasively monitor vimentin cytoskeleton in your cells.
|Applications||Trace endogenous vimentin in real-time|
Live cell imaging
Monitor epithelial-mesenchymal transition in live cells
|Specificity||Vimentin intermediate filament protein, tested in human, hamster & dog cells|
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Sequence||With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.
Methods Cell Biol
Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.