ChromoTek Vimentin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.




IF, Live cell imaging

Vector type

Mammalian expression vector



Cat no : vcg


Chromobody, Vimentin Chromobody

Product Information

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

DescriptionThe Vimentin-Chromobody visualizes endogenous Vimentin in real time.

• Easily transfect your cells with the Vimentin-Chromobody plasmid

• Use Vimentin-Chromobody as a marker of vimentin cytoskeleton

• Monitor dynamics of vimentin intermediate filaments in live cells

• Vimentin-Chromobody does not affect cell viability

• The binding of Vimentin-Chromobody does not influence cell viability or cell motility and thus offers you the unique possibility to non-invasively monitor vimentin cytoskeleton in your cells.

ApplicationsTrace endogenous vimentin in real-time
Live cell imaging
Monitor epithelial-mesenchymal transition in live cells
SpecificityVimentin intermediate filament protein, tested in human, hamster & dog cells
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SequenceWith the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C



Cancer Res

Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.

Authors - Julia Maier

Methods Cell Biol

Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.

Authors - Catherine S Schrankel