ChromoTek Vimentin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Vimentin

Publications

2

Cat no : vcg

Synonyms

Chromobody, Vimentin Chromobody

---

Validation Data Gallery

• 

  Visualize vimentin cytoskeleton with the Vimentin-Chromobody in real time in your cell line of interest

• 

  Vimentin-Chromobody visualizes Vimentin filaments in live cells

---

Product Information

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

• Applications: Live cell imaging
• Specificity: Vimentin intermediate filament protein, tested in human, hamster & dog cells
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagGFP2
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-Vimentin single domain antibody (sdAb) fragment
• Sequence: With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Methods Cell Biol; Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.; Authors - Catherine S Schrankel
IF	Cancer Res; Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.; Authors - Julia Maier