Cat no : vcg
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.
|Applications||Live cell imaging|
|Specificity||Vimentin intermediate filament protein, tested in human, hamster & dog cells|
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Encoded Nanobody/ VHH||Monoclonal anti-Vimentin single domain antibody (sdAb) fragment|
|Sequence||With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
Methods Cell Biol
Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.
Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.