Total antibody (IgG and IgM) SARS-CoV-2 N protein ELISA Kit
Cat no : KE30005
N protein, nuclepcapsid phosphoprotein, SARS-CoV-2 N protein
KE30005 is a qualitative measurement of total antibody (IgG and IgM) to SARS-COV-2 N protein in serum or plasma. The principle of the kit is sandwich ELISA. anti-human antibody has been pre- coated onto microplate well. The samples or standard are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated anti-N protein is added to each well. Producing an complex “anti–human antibody and sample and SARS-CoV-2 N protein-HRP conjugated”. after incubation the wells are washed, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
|Product name||Total antibody (IgG and IgM) SARS-CoV-2 N protein ELISA Kit|
|Tests||1 X 96 well plate|
|Sample type||Serum, Plasma|
|Tested applications||Capture ELISA|
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways . Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. COVID-19 antibodies can be produced by a host immune system following exposure to SARS-CoV-2. IgG and IgM antibodies are also known as immunoglobulins IgG and IgM, respectively, and are among the antibody isotypes produced by vertebrate immune systems. The ELISA microplate is coated with the SARS-CoV-2 nucleocapsid (N) protein. The coated N protein binds with COVID-19 IgM N antibodies in the serum sample.
|Storage Instructions||All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.|
|Synonyms||N protein, nuclepcapsid phosphoprotein, SARS-CoV-2 N protein|