Total antibody (IgG and IgM) SARS-CoV-2 S-RBD ELISA Kit

Range

6.25-200 ng/mL

Cat no : KE30006

Synonyms

SARS-CoV-2 S-RBD, spike protein



Product Information

KE30006 is a qualitative measurement of total antibody (IgG and IgM) to SARS-COV-2 S-RBD protein in serum or plasma. The principle of the kit is sandwich ELISA. anti-human antibody has been pre-coated onto microplate well. The samples or standard are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated S-RBD protein is added to each well. Producing an complex “anti–human antibody and sample and SARS-CoV-2 S-RBD protein-HRP conjugated”. after incubation the wells are washed, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Total antibody (IgG and IgM) SARS-CoV-2 S-RBD ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Capture
Sensitivity N/A
Range 6.25-200 ng/mL
Reacted Species
Tested applications Capture ELISA

Background Information

A promising target for both diagnosis and therapeutics treatments of the new disease named COVID-19 is the coronavirus (CoV) spike (S) glycoprotein. The spike protein, which is responsible for the “corona” (Latin word for crown) appearance in all coronaviruses, is a type I glycoprotein that has an especial role in the interaction between the virus and the host cell. This protein attaches itself to specific cellular receptors and suffers a conformational change that enables the fusion of the virus and the cell. Studies have shown that the SARS-CoV-2’s S RBD protein interacts strongly with the Angiotensin-converting enzyme 2 (ACE2). S-RBD protein in order to enlighten the binding epitopes of these Abs. Because of the conservation of S-RBD protein sequence and its strong immunogenicity, the S-RBD protein of coronavirus is chosen as a diagnostic tool. COVID-19 antibodies can be produced by a host immune system following exposure to SARS-CoV-2. IgM antibodies are also known as immunoglobulins IgG and IgM, respectively, and are among the antibody isotypes produced by vertebrate immune systems.The ELISA microplate is coated with the SARS-CoV-2 S-RBD protein. The coated S-RBD protein binds with COVID-19 IgM S-RBD antibodies in the serum sample.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms SARS-CoV-2 S-RBD, spike protein