Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20778-1-AP targets WDR24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14774 Product name: Recombinant human WDR24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 440-790 aa of BC008025 Sequence: LGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLPLMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLLGDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGSEADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVLGERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCSHCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCPAGCGHLCEYS Predict reactive species |
| Full Name | WD repeat domain 24 |
| Calculated Molecular Weight | 920 aa, 102 kDa |
| Observed Molecular Weight | 102 kDa, 88 kDa |
| GenBank Accession Number | BC008025 |
| Gene Symbol | WDR24 |
| Gene ID (NCBI) | 84219 |
| RRID | AB_10696183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96S15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WDR24 antibody 20778-1-AP | Download protocol |
| IHC protocol for WDR24 antibody 20778-1-AP | Download protocol |
| IP protocol for WDR24 antibody 20778-1-AP | Download protocol |
| WB protocol for WDR24 antibody 20778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Sestrin1 antibody (Cat# 20778-1-AP) has 32 citations across 21 journals, including Nature, Science, Molecular Cell, Nature Communications, Cell Reports, and PNAS. Research fields span mTORC1 signaling, amino acid/nutrient sensing, GATOR2 complex regulation, tumorigenesis, metabolic regulation, immune response, and autophagy. Primary applications include WB, IP, CoIP, and IF.
| Species | Application | Title |
|---|---|---|
