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  • KD/KO Validated

WDR24 Polyclonal antibody

WDR24 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20778-1-AP

Synonyms

C16orf21, JFP7, WD repeat domain 24, WDR24



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20778-1-AP is WDR24 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR24 fusion protein Ag14774
Full Name WD repeat domain 24
Calculated molecular weight 920 aa, 102 kDa
Observed molecular weight 102 kDa, 88 kDa
GenBank accession numberBC008025
Gene symbol WDR24
Gene ID (NCBI) 84219
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

Protocols

Product Specific Protocols
WB protocol for WDR24 antibody 20778-1-APDownload protocol
IHC protocol for WDR24 antibody 20778-1-APDownload protocol
IF protocol for WDR24 antibody 20778-1-APDownload protocol
IP protocol for WDR24 antibody 20778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Physiol Endocrinol Metab

Evidence for a Role for Sestrin1 in Mediating Leucine-Induced Activation of mTORC1 in Skeletal Muscle.

Authors - Dandan Xu
humanWB,IF

Methods

CRISPR/Cas9-mediated generic protein tagging in mammalian cells.

Authors - Fabian M B Thöne
humanIF

J Cell Sci

Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.

Authors - Melpomeni Platani
humanWB

Nature

SZT2 dictates GATOR control of mTORC1 signalling.

Authors - Min Peng
  • KO Validated
WB,IP

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen