WDR45 Antibody

WDR45 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19194-1-AP

Synonyms

JM5, WD repeat domain 45, WDR45, WDRX1, WDRXI4, WIPI 4, WIPI4



Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

19194-1-AP targets WDR45 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR45 fusion protein Ag6741
Full Name WD repeat domain 45
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession number BC000464
Gene symbol WDR45
Gene ID (NCBI) 11152
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WDR45 antibody 19194-1-APDownload protocol
IHC protocol for WDR45 antibody 19194-1-APDownload protocol
IF protocol for WDR45 antibody 19194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Med Genet

Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.

Authors - Hongbo Chen