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  • KD/KO Validated

WDR45/WIPI4 Polyclonal antibody

WDR45/WIPI4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19194-1-AP

Synonyms

JM5, WD repeat domain 45, WDR45, WDRX1, WDRXI4, WIPI 4, WIPI4



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19194-1-AP targets WDR45/WIPI4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR45/WIPI4 fusion protein Ag6741
Full Name WD repeat domain 45
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000464
Gene symbol WDR45
Gene ID (NCBI) 11152
RRIDAB_2215404
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).

Protocols

Product Specific Protocols
WB protocol for WDR45/WIPI4 antibody 19194-1-APDownload protocol
IHC protocol for WDR45/WIPI4 antibody 19194-1-APDownload protocol
IF protocol for WDR45/WIPI4 antibody 19194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
mouseWB,IP

Sci Adv

Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.

Authors - Alexander S Häusl
  • KD Validated
humanWB

Int J Mol Sci

Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.

Authors - Hye Eun Lee
  • KD Validated
humanWB

J Neurochem

A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation

Authors - Luisa Aring
  • KO Validated
humanWB

Am J Med Genet A

Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration.

Authors - Jae-Hyeok Lee
humanWB

Eur J Med Genet

Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.

Authors - Hongbo Chen