WDR45/WIPI4 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 19194-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WDR45/WIPI4 antibody (19194-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

WDR45/WIPI4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19194-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IP: See 1 publications below

Product Information

19194-1-AP targets WDR45/WIPI4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR45/WIPI4 fusion protein Ag6741
• Full Name: WD repeat domain 45
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000464
• Gene Symbol: WDR45
• Gene ID (NCBI): 11152
• RRID: AB_2215404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB,IP	Sci Adv; Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.; Authors - Alexander S Häusl ; KD Validated
human	WB	Int J Mol Sci; Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.; Authors - Hye Eun Lee ; KD Validated
human	WB	J Neurochem; A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation; Authors - Luisa Aring ; KO Validated
human	WB	Am J Med Genet A; Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration.; Authors - Jae-Hyeok Lee
human	WB	Eur J Med Genet; Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.; Authors - Hongbo Chen