WDR45 Polyclonal antibody

WDR45 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19194-1-AP

Synonyms

JM5, WD repeat domain 45, WDR45, WDRX1, WDRXI4, WIPI 4, WIPI4

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 19194-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

19194-1-AP targets WDR45 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR45 fusion protein Ag6741
• Full Name: WD repeat domain 45
• Calculated molecular weight: 40 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC000464
• Gene symbol: WDR45
• Gene ID (NCBI): 11152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Eur J Med Genet; Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.; Authors - Hongbo Chen