WHAMM Polyclonal antibody

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  WB analysis using 31572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31572-1-AP (WHAMM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WHAMM Polyclonal Antibody for WB, ELISA

Cat No. 31572-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

WHDC1, KIAA1971

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Tested Applications

• Positive WB detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31572-1-AP targets WHAMM in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WHAMM fusion protein Ag36160
• Full Name: WAS protein homolog associated with actin, golgi membranes and microtubules
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC167774
• Gene Symbol: WHAMM
• Gene ID (NCBI): 123720
• RRID: AB_3670039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TF30
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WHAMM (WASP homolog associated with actin, golgi membranes and microtubules), also known as WHDC1. It is expected to be located in the cytoplasm, which is enriched in brain, lung, heart, colon and kidney. WHAMM is involved in Golgi membrane association, microtubule binding, and actin nucleation as a nucleation-promoting factor, which activates the actin-related protein 2/3 complex (the Arp2/ 3 complex) (PMID: 23160625). It is reported that the nucleation-promoting factor (NPF) WHAMM recruits and activates the Arp2/3 complex for actin assembly at sites of autophagosome formation on the ER (PMID: 26291929). The molecular weight of WHAMM is 90 kDa.