WIPF1 Polyclonal antibody, PBS Only

• 

  WB analysis using 22047-1-AP (same clone as 22047-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 22047-1-AP (same clone as 22047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22047-1-PBS in a different storage buffer formulation.

WIPF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22047-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

---

Product Information

22047-1-PBS targets WIPF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WIPF1 fusion protein Ag16975
• Full Name: WAS/WASL interacting protein family, member 1
• Calculated Molecular Weight: 403aa,40 kDa; 503aa,51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC110288
• Gene Symbol: WIPF1
• Gene ID (NCBI): 7456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43516
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).