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  • KD/KO Validated

WNT2 Monoclonal antibody

WNT2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (1)







Cat no : 66656-1-Ig


Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, Jurkat cells, RAW 264.7 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66656-1-Ig targets WNT2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WNT2 fusion protein Ag25185
Full Name wingless-type MMTV integration site family member 2
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC029854
Gene Symbol WNT2
Gene ID (NCBI) 7472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.


Product Specific Protocols
WB protocol for WNT2 antibody 66656-1-IgDownload protocol
IF protocol for WNT2 antibody 66656-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer

Authors - Atish Mohanty
  • KD Validated

Cell Mol Life Sci

α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.

Authors - Zewu Zhu

J Surg Res

Expression Analysis of ACSL5 and Wnt2B in Human Congenital Pulmonary Airway Malformations.

Authors - Yuan Qu

Int J Endocrinol

Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway

Authors - Meizhong Peng

Int J Oncol

FUT2 promotes the tumorigenicity and metastasis of colorectal cancer cells via the Wnt/β‑catenin pathway

Authors - Peng Liu