|Positive WB detected in||HeLa cells, HSC-T6 cells, Jurkat cells, RAW 264.7 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, MCF-7 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66656-1-Ig targets WNT2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||WNT2 fusion protein Ag25185|
|Full Name||wingless-type MMTV integration site family member 2|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC029854|
|Gene ID (NCBI)||7472|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.
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