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  • KD/KO Validated

WNT5A/B Polyclonal antibody

WNT5A/B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55184-1-AP

Synonyms

hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B, WNT5A/B, WNT5B



Tested Applications

Positive WB detected inmouse heart tissue, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55184-1-AP targets WNT5A/B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name wingless-type MMTV integration site family, member 5A
Calculated molecular weight 42 kDa
Observed molecular weight 38 kDa
GenBank accession numberNM_003392
Gene symbol WNT5A
Gene ID (NCBI) 7474
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptidesThis antibody can recognize WNT5A and WNT5B.

Protocols

Product Specific Protocols
WB protocol for WNT5A/B antibody 55184-1-APDownload protocol
IHC protocol for WNT5A/B antibody 55184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Genome Biol

Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma.

Authors - Jianyu Yang

Biomaterials

Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.

Authors - Yonglin Yu
mouseWB

J Neuroinflammation

Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model

Authors - Yitian Lu
mouseWB

Front Cell Dev Biol

Macrophage-Mediated Bone Formation in Scaffolds Modified With MSC-Derived Extracellular Matrix Is Dependent on the Migration Inhibitory Factor Signaling Pathway.

Authors - Moyuan Deng
humanWB

Stem Cell Res Ther

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway.

Authors - Zheng-Liang Shi
chickenWB

Toxicol Appl Pharmacol

Perfluorooctanoic acid-induced toxicities in chicken embryo primary cardiomyocytes: Roles of PPAR alpha and Wnt5a/Frizzled2.

Authors - Na Lv

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Darin (Verified Customer) (11-02-2017)

No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Lung