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WNT5A/B Polyclonal antibody

WNT5A/B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (3)





Cat no : 55184-1-AP


hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B, WNT5A/B, WNT5B

Tested Applications

Positive WB detected inmouse heart tissue, HUVEC cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55184-1-AP targets WNT5A/B in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name wingless-type MMTV integration site family, member 5A
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberNM_003392
Gene Symbol WNT5A
Gene ID (NCBI) 7474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.


Product Specific Protocols
WB protocol for WNT5A/B antibody 55184-1-APDownload protocol
IHC protocol for WNT5A/B antibody 55184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Darin (Verified Customer) (11-02-2017)

No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Lung