WNT5A/B Polyclonal antibody

• Featured Product
• KD/KO Validated

WNT5A/B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (1)

Cat no : 55184-1-AP

Synonyms

WNT5A, WNT5B, WNT5A B, Protein Wnt-5a, Protein Wnt 5a

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Validation Data Gallery

• 

  WB analysis using 55184-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55184-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 55184-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 55184-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HUVEC cells, PC-3 cells
• Positive IHC detected in: human lung cancer tissue, human tonsillitis tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 44 publications below
• IHC: See 8 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

55184-1-AP targets WNT5A/B in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: wingless-type MMTV integration site family, member 5A
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_003392
• Gene Symbol: WNT5A
• Gene ID (NCBI): 7474
• RRID: AB_2881285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IHC	Genome Biol; Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma.; Authors - Jianyu Yang
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
		Biomaterials; Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.; Authors - Yonglin Yu
human	WB	Nanoscale; An assembly-inducing PDC enabling the efficient nuclear delivery of nucleic acid for cancer stem-like cell suppression; Authors - Dongyuan Wang
mouse	WB,IF,RIP	Arch Med Res; RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway; Authors - Zhao Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Darin (Verified Customer) (11-02-2017)

No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Lung

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