Validation Data Gallery
|Positive WB detected in||mouse heart tissue, PC-3 cells|
|Positive IHC detected in||human lung cancer tissue, human tonsillitis tissue, human thyroid cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55184-1-AP targets WNT5A/B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Full Name||wingless-type MMTV integration site family, member 5A|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||NM_003392|
|Gene ID (NCBI)||7474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptidesThis antibody can recognize WNT5A and WNT5B.
Integrated genomic and transcriptomic analysis reveals unique characteristics of hepatic metastases and pro-metastatic role of complement C1q in pancreatic ductal adenocarcinoma.
Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.
Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model
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Macrophage-Mediated Bone Formation in Scaffolds Modified With MSC-Derived Extracellular Matrix Is Dependent on the Migration Inhibitory Factor Signaling Pathway.
Stem Cell Res Ther
Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway.
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Perfluorooctanoic acid-induced toxicities in chicken embryo primary cardiomyocytes: Roles of PPAR alpha and Wnt5a/Frizzled2.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Darin (Verified Customer) (11-02-2017)
No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.