Validation Data Gallery
|Positive WB detected in||mouse heart tissue, HUVEC cells, PC-3 cells|
|Positive IHC detected in||human lung cancer tissue, human tonsillitis tissue, human thyroid cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55184-1-AP targets WNT5A/B in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Rabbit / IgG|
|Full Name||wingless-type MMTV integration site family, member 5A|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||38 kDa|
|GenBank accession number||NM_003392|
|Gene ID (NCBI)||7474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptidesThis antibody can recognize WNT5A and WNT5B.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Darin (Verified Customer) (11-02-2017)
No signal in lung epithelial cells from mice. However, it is possible that the protein is not expressed in lung epithelial cells.