WNT7A/B Polyclonal antibody

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• WNT7A/B Polyclonal antibody (10605-1-AP)

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• KD/KO Validated

WNT7A/B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10605-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

WNT7B, WNT7A, Protein Wnt 7a, Protein Wnt-7a

• 

  WB analysis using 10605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 10605-1-AP

  IP result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:4000) with mouse kidney tissue lysate 2280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse placenta using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 10605-1-AP (WNT7A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse placenta using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 10605-1-AP (WNT7A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10605-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (10605-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10605-1-AP targets WNT7A/B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0874

  Product name: Recombinant human WNT7A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC008811

  Sequence: MNRKARRCLGHLFLSLGMVYLRIGGFSSVVALGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEEDPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK

• Full Name: wingless-type MMTV integration site family, member 7A
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 30-39 kDa
• GenBank Accession Number: BC008811
• Gene Symbol: WNT7A
• Gene ID (NCBI): 7476
• RRID: AB_2215625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00755
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Kidney Int; Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis; Authors - Dong Zhou
human	WB	Autophagy; WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway.; Authors - Danqiong Liu
human	IF	Nat Commun; Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis.; Authors - Wei Wei
mouse	WB	Cell Rep Med; SIRT1 mediates brain metabolic and developmental consequences of methionine synthase deficiency in inborn errors of cobalamin metabolism.; Authors - Karim Matmat
human	WB	Sci Adv; Modulation of blood-tumor barrier transcriptional programs improves intratumoral drug delivery and potentiates chemotherapy in GBM; Authors - Jorge L Jimenez-Macias
mouse	WB	Free Radic Biol Med; Aldehyde dehydrogenase ALDH3A1 rescues cigarette smoke-induced emphysema by conferring alveolar type 2 to type 1 cell transition.; Authors - Jiajing Cui