WNT7A/B Antibody

WNT7A/B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10605-1-AP

Synonyms

Protein Wnt 7a, WNT7A



Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10605-1-AP targets WNT7A/B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT7A/B fusion protein Ag0874
Full Name wingless-type MMTV integration site family, member 7A
Calculated molecular weight 39 kDa
Observed molecular weight 39-43 kDa
GenBank accession number BC008811
Gene symbol WNT7A
Gene ID (NCBI) 7476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

Protocols

Product Specific Protocols
WB protocol for WNT7A/B antibody 10605-1-APDownload protocol
IHC protocol for WNT7A/B antibody 10605-1-APDownload protocol
IF protocol for WNT7A/B antibody 10605-1-APDownload protocol
IP protocol for WNT7A/B antibody 10605-1-APDownload protocol
FC protocol for WNT7A/B antibody 10605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Neurol Res

Wnt signaling pathway is involved in the pathogenesis of amyotrophic lateral sclerosis in adult transgenic mice.

Authors - Chen Yanchun Y
WB

Oncol Rep

RNA-binding motif protein 5 negatively regulates the activity of Wnt/β-catenin signaling in cigarette smoke-induced alveolar epithelial injury.

Authors - Yu-Qiu Hao