|Positive WB detected in||mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10605-1-AP targets WNT7A/B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WNT7A/B fusion protein Ag0874|
|Full Name||wingless-type MMTV integration site family, member 7A|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||39-43 kDa|
|GenBank accession number||BC008811|
|Gene ID (NCBI)||7476|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.
|Product Specific Protocols|
|WB protocol for WNT7A/B antibody 10605-1-AP||Download protocol|
|IHC protocol for WNT7A/B antibody 10605-1-AP||Download protocol|
|IF protocol for WNT7A/B antibody 10605-1-AP||Download protocol|
|IP protocol for WNT7A/B antibody 10605-1-AP||Download protocol|
|FC protocol for WNT7A/B antibody 10605-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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