WNT7A/B Polyclonal antibody

WNT7A/B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10605-1-AP

Synonyms

Protein Wnt 7a, WNT7A, WNT7A/B, WNT7B

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Validation Data Gallery

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  WB analysis using 10605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10605-1-AP

  IP Result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10605-1-AP (WNT7A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10605-1-AP (WNT7A antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10605-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10605-1-AP (WNT7A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10605-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug WNT7A antibody (10605-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

10605-1-AP targets WNT7A/B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT7A/B fusion protein Ag0874
• Full Name: wingless-type MMTV integration site family, member 7A
• Calculated molecular weight: 39 kDa
• Observed molecular weight: 35-39 kDa
• GenBank accession number: BC008811
• Gene symbol: WNT7A
• Gene ID (NCBI): 7476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

Publications

Species	Application	Title
human	WB, IHC	Front Oncol; The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.; Authors - Chun Tang
human	WB,IHC	Oncol Rep; Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma.; Authors - Bo Jia ; KD Validated
mouse	WB	Behav Brain Res; OGDHL ameliorates cognitive impairment and Alzheimer's disease-like pathology via activating Wnt/β-catenin signaling in Alzheimer's disease mice.; Authors - Li Yao
	WB	Oncol Rep; RNA-binding motif protein 5 negatively regulates the activity of Wnt/β-catenin signaling in cigarette smoke-induced alveolar epithelial injury.; Authors - Yu-Qiu Hao
mouse	WB,IHC	Neurol Res; Wnt signaling pathway is involved in the pathogenesis of amyotrophic lateral sclerosis in adult transgenic mice.; Authors - Chen Yanchun Y
mouse	WB	NAR Cancer; EVI1 activates tumor-promoting transcriptional enhancers in pancreatic cancer.; Authors - Hwa-Ryeon Kim