|Positive WB detected in||K-562 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, A431 cells, MCF-7 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IF detected in||K-562 cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12609-1-AP targets WT1 in WB, IP, IF, FC, chIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, goat, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WT1 fusion protein Ag3337|
|Full Name||Wilms tumor 1|
|Calculated molecular weight||449 aa, 49 kDa, 57 kDa|
|Observed molecular weight||52-55 kDa|
|GenBank accession number||BC032861|
|Gene ID (NCBI)||7490|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.
Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.
Cell Death Dis
Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.
Genome profiling of mismatch repair genes in eight types of tumors.
Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.
Mol Med Rep
Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.
WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sandrine (Verified Customer) (12-17-2021)
clean signal for IP and western blot. Good relative enrichment for ChIP assays.
K (Verified Customer) (12-20-2020)
This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.