|Positive WB detected in||human kidney tissue, A431 cells, human liver tissue, K-562 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
12609-1-AP targets WT1 in WB, IP, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WT1 fusion protein Ag3337|
|Full Name||Wilms tumor 1|
|Calculated molecular weight||449 aa, 49 kDa, 57 kDa|
|Observed molecular weight||52-55 kDa|
|GenBank accession number||BC032861|
|Gene ID (NCBI)||7490|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.
Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.
Cell Death Dis
Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.
Cell Physiol Biochem
Analysis of Circulating Tumor Cells in Ovarian Cancer and Their Clinical Value as a Biomarker.
Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.
YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.
EMBO Mol Med
Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (12-20-2020)
This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.