• Featured Product
  • KD/KO Validated

WT1 Polyclonal antibody

WT1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, FC, chIP

Conjugate

Unconjugated

Cat no : 12609-1-AP

Synonyms

GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33



Tested Applications

Positive WB detected inK-562 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, A431 cells, MCF-7 cells
Positive IP detected inmouse kidney tissue
Positive IF detected inK-562 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12609-1-AP targets WT1 in WB, IP, IF, FC, chIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, goat, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WT1 fusion protein Ag3337
Full Name Wilms tumor 1
Calculated molecular weight 449 aa, 49 kDa, 57 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC032861
Gene symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.

Protocols

Product Specific Protocols
WB protocol for WT1 antibody 12609-1-APDownload protocol
IF protocol for WT1 antibody 12609-1-APDownload protocol
IP protocol for WT1 antibody 12609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,chIP

JCI Insight

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

Authors - Vishwaraj Sontake
  • KO Validated
mouseWB

Cell Death Dis

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Authors - Kewei Xie
humanWB,IHC

Cell Cycle

Genome profiling of mismatch repair genes in eight types of tumors.

Authors - Siqi Wang
  • KD Validated
humanIF

STAR Protoc

Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.

Authors - Julie Bejoy
mouseWB

Mol Med Rep

Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.

Authors - Zhengming Zhu
chickenWB

Gene

WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.

Authors - Shuixin Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sandrine (Verified Customer) (12-17-2021)

clean signal for IP and western blot. Good relative enrichment for ChIP assays.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation, ChIP
  • Cell Tissue Type: kidney tissue/Podocytes
FH

K (Verified Customer) (12-20-2020)

This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.

  • Applications: Western Blot, Immunohistochemistry
  • Cell Tissue Type: mouse liver tissues. rat liver and human liver cells