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  • KD/KO Validated

WT1 Polyclonal antibody

WT1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12609-1-AP

Synonyms

GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33



Tested Applications

Positive WB detected inhuman kidney tissue, A431 cells, human liver tissue, K-562 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12609-1-AP targets WT1 in WB, IP, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WT1 fusion protein Ag3337
Full Name Wilms tumor 1
Calculated molecular weight 449 aa, 49 kDa, 57 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC032861
Gene symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.

Protocols

Product Specific Protocols
WB protocol for WT1 antibody 12609-1-APDownload protocol
IP protocol for WT1 antibody 12609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,chIP

JCI Insight

Wilms' tumor 1 drives fibroproliferation and myofibroblast transformation in severe fibrotic lung disease.

Authors - Vishwaraj Sontake
  • KO Validated
mouseWB

Cell Death Dis

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Authors - Kewei Xie
humanIHC

Cell Physiol Biochem

Analysis of Circulating Tumor Cells in Ovarian Cancer and Their Clinical Value as a Biomarker.

Authors - Xuehui Zhang
humanIF

Elife

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

Authors - Jianlin Liang
humanWB, IHC

Oncogene

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Authors - G Hua
mousechIP

EMBO Mol Med

Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.

Authors - Rajesh K Kasam