Tested Applications
| Positive WB detected in | RAW 264.7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, HeLa cells, C6 cells, NIH/3T3 cells, Raji cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10037-1-Ig targets XIAP in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0022 Product name: Recombinant human XIAP protein Source: e coli.-derived, Unknow Tag: N/A Domain: XIAP of BC032729 Sequence: Predict reactive species |
| Full Name | X-linked inhibitor of apoptosis |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 45-57 kDa |
| GenBank Accession Number | BC032729 |
| Gene Symbol | XIAP |
| Gene ID (NCBI) | 331 |
| RRID | AB_2215009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P98170 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for XIAP antibody 10037-1-Ig | Download protocol |
| WB protocol for XIAP antibody 10037-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-XIAP antibody (Cat# 10037-1-Ig) has accumulated 58 citations across more than 45 distinct journals, including Cell, Nature Communications, Cancer Research, EMBO Reports, Science Advances, Cell Death & Differentiation, Clinical and Translational Medicine, and Molecular Therapy Oncolytics. Cited research spans apoptosis regulation, multiple cancer types (hepatocellular, prostate, breast, lung, colorectal, pancreatic, glioblastoma, melanoma, medulloblastoma), inflammatory diseases, neurodegeneration, diabetes, liver fibrosis, osteoarthritis, and novel drug discovery. Primary applications are Western blot and immunohistochemistry, with studies conducted in human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Cell Rift Valley fever virus coordinates the assembly of a programmable E3 ligase to promote viral replication | ||
Cancer Res DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer | ||
Cancer Res An ATM/TRIM37/NEMO axis counteracts genotoxicity by activating nuclear-to-cytoplasmic NF-κB signaling. | ||
Nat Commun Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer. | ||
Nat Commun The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. | ||
Exp Mol Med PBK drives PARP inhibitor resistance through the TRIM37/NFκB axis in ovarian cancer |
