XIAP Polyclonal antibody

• Featured Product
• KD/KO Validated

XIAP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 10037-1-Ig

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

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Validation Data Gallery

• 

  WB analysis using 10037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10037-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10037-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10037-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10037-1-Ig(XIAP antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, Jurkat cells, HepG2 cells, HeLa cells, COLO 320 cells, C6 cells, NIH/3T3 cells, Raji cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 8 publications below

Product Information

10037-1-Ig targets XIAP in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XIAP fusion protein Ag0022
• Full Name: X-linked inhibitor of apoptosis
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 45-57 kDa
• GenBank accession number: BC032729
• Gene symbol: XIAP
• Gene ID (NCBI): 331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.; Authors - Weimei Tang
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan
human	WB	Cell Death Dis; Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.; Authors - Jingru Huangfu
human	WB	Acta Pharmacol Sin; Aspirin modulates succinylation of PGAM1K99 to restrict the glycolysis through NF-κB/HAT1/PGAM1 signaling in liver cancer.; Authors - Yu-Fei Wang
human	WB	J Agric Food Chem; β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.; Authors - Heshan Xu
human	WB	Exp Ther Med; PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.; Authors - Xiaohui Xu

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