|Positive WB detected in||RAW 264.7 cells, Jurkat cells, HepG2 cells, HeLa cells, COLO 320 cells, C6 cells, NIH/3T3 cells, Raji cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10037-1-Ig targets XIAP in WB, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XIAP fusion protein Ag0022|
|Full Name||X-linked inhibitor of apoptosis|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||45-57 kDa|
|GenBank accession number||BC032729|
|Gene ID (NCBI)||331|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.
The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.
Int J Nanomedicine
Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.
Cell Death Dis
Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.
J Agric Food Chem
β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.
Exp Ther Med
PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.
Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.