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- KD/KO Validated
XRCC4 Polyclonal antibody
XRCC4 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IP, IHC
Conjugate
Unconjugated
Cat no : 15817-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, COLO 320 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human breast cancer tissue, rat testis tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 1 publications below |
Product Information
15817-1-AP targets XRCC4 in WB, IP, IHC applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | XRCC4 fusion protein Ag8606 |
Full Name | X-ray repair complementing defective repair in Chinese hamster cells 4 |
Calculated molecular weight | 310 aa, 35 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC005259 |
Gene symbol | XRCC4 |
Gene ID (NCBI) | 7518 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.
Protocols
Product Specific Protocols | |
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WB protocol for XRCC4 antibody 15817-1-AP | Download protocol |
IHC protocol for XRCC4 antibody 15817-1-AP | Download protocol |
IP protocol for XRCC4 antibody 15817-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biosci Rep Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells.
| ||
FEBS Lett RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway. | ||
J Cancer Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma. | ||
PLoS One 5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation. | ||
Toxicol Appl Pharmacol Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. | ||
Cell Death Differ LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair. |