XRCC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

XRCC4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15817-1-AP

Synonyms

DNA repair protein XRCC4, XRCC4

---

Validation Data Gallery

• 

  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  WB analysis of HepG2 using 15817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15817-1-AP

  IP Result of anti-XRCC4 (IP:15817-1-AP, 4ug; Detection:15817-1-AP 1:800) with HepG2 cells lysate 3200ug.

---

Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue, rat testis tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

15817-1-AP targets XRCC4 in WB, IP, IHC,ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XRCC4 fusion protein Ag8606
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Calculated molecular weight: 310 aa, 35 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC005259
• Gene symbol: XRCC4
• Gene ID (NCBI): 7518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Publications

Species	Application	Title
human	WB,IHC	Biosci Rep; Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells.; Authors - Yuqing Wen ; KD Validated
human	WB	FEBS Lett; RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.; Authors - Yiran Zhong
human	WB	PLoS One; 5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.; Authors - Wei Jiang
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong
human	WB	Cell Death Differ; LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.; Authors - Dan Wang
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou