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  • KD/KO Validated

XRCC4 Polyclonal antibody

XRCC4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (1)





Cat no : 15817-1-AP


DNA repair protein XRCC4, XRCC4

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, COLO 320 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, rat testis tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15817-1-AP targets XRCC4 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8606
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated Molecular Weight 310 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005259
Gene Symbol XRCC4
Gene ID (NCBI) 7518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.


Product Specific Protocols
WB protocol for XRCC4 antibody 15817-1-APDownload protocol
IHC protocol for XRCC4 antibody 15817-1-APDownload protocol
IP protocol for XRCC4 antibody 15817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Authors - Guijie Guo
  • KD Validated

Cancer Res

Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.

Authors - Qin Zhou

Cell Death Differ

LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.

Authors - Dan Wang

Toxicol Appl Pharmacol

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Authors - Hui Dong

PLoS One

5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.

Authors - Wei Jiang

J Cancer

Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.

Authors - Yi Liu