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  • KD/KO Validated

XRCC4 Polyclonal antibody

XRCC4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15817-1-AP

Synonyms

DNA repair protein XRCC4, XRCC4



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, rat testis tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15817-1-AP targets XRCC4 in WB, IP, IHC,ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8606
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated molecular weight 310 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005259
Gene symbol XRCC4
Gene ID (NCBI) 7518
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Protocols

Product Specific Protocols
WB protocol for XRCC4 antibody 15817-1-APDownload protocol
IHC protocol for XRCC4 antibody 15817-1-APDownload protocol
IP protocol for XRCC4 antibody 15817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biosci Rep

Silencing of XRCC4 increases radiosensitivity of triple-negative breast cancer cells.

Authors - Yuqing Wen
  • KD Validated
humanWB

FEBS Lett

RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.

Authors - Yiran Zhong
humanWB

PLoS One

5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.

Authors - Wei Jiang
humanWB

Toxicol Appl Pharmacol

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Authors - Hui Dong
humanWB

Cell Death Differ

LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.

Authors - Dan Wang
humanWB

Nat Commun

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Authors - Guijie Guo
  • KD Validated