XYLT1 Recombinant antibody

• 

  WB analysis using 85008-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85008-1-RR (XYLT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

XYLT1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85008-1-RR

Clone No.242315A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242315A12, EC:2.4.2.26, Peptide O xylosyltransferase 1, Peptide O-xylosyltransferase 1, XT-I

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85008-1-RR targets XYLT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: xylosyltransferase I
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 110~120 kDa
• GenBank Accession Number: NM_022166
• Gene Symbol: XYLT1
• Gene ID (NCBI): 64131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86Y38
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.