YAP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

YAP1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

10

CloneNo.

3A7A9

Reviews (1)

Cat no : 66900-1-Ig

Synonyms

65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

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Validation Data Gallery

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  WB analysis using 66900-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66900-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66900-1-Ig (YAP1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, NIH/3T3 cells, MCF-7 cells, HUVEC cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

66900-1-Ig targets YAP1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: YAP1 fusion protein Ag28194
• Full Name: Yes-associated protein 1, 65kDa
• Calculated molecular weight: 504 aa, 54 kDa
• Observed molecular weight: 70 kDa
• GenBank accession number: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65-70 kDa. (PMID: 26695440)

Publications

Species	Application	Title
human	WB	Am J Transl Res; VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.; Authors - Adheesh Bhandari
human	WB	Cell Death Dis; CLDN6 promotes tumor progression through the YAP1-snail1 axis in gastric cancer.; Authors - Site Yu
human	WB,IHC,IF	Oncotarget; Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.; Authors - Xiaodong Zhang
human	WB,IHC	J Exp Clin Cancer Res; Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.; Authors - Ming-Xin Cao
human	WB,IHC	Cell Death Dis; CircATRNL1 promotes epithelial-mesenchymal transition in endometriosis by upregulating Yes-associated protein 1 in vitro.; Authors - Dandan Wang
mouse	WB	Immunol Lett; Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.; Authors - Peiyu Gao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (01-29-2019)

Coverslips were fixed with 4%PFA+0.25% Triton-X-100. Primary staining was 1:500, secondary was 1:750 with rockland 488 anti-mouse overnight at 4C.Left panel: Control PANC1 cells.Right panel: PANC1 cells with YAP silencing

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: PANC1

YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in PANC1 (Cat no:66900-1-Ig)