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  • KD/KO Validated

YBX1 Polyclonal antibody

YBX1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 20339-1-AP


BP 8, CBF A, CSDA2, CSDB, DBPB, DNA binding protein B, EFI A, Enhancer factor I subunit A, MDR NF1, NSEP 1, NSEP1, Y box binding protein 1, Y box transcription factor, YB 1, YB1, YBX1

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, NIH/3T3 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human placenta tissue, human breast cancer tissue, human prostate cancer tissue, human tonsillitis tissue, mouse colon tissue, rat colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20339-1-AP targets YBX1 in WB, IP, IF, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YBX1 fusion protein Ag14129
Full Name Y box binding protein 1
Calculated Molecular Weight 324 aa, 36 kDa
Observed Molecular Weight 36-56 kDa
GenBank Accession NumberBC010430
Gene Symbol YBX1
Gene ID (NCBI) 4904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also, it involves the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. The calculated molecular weight of YBX1 is 36 kDa, but modified YBX1 is about 36-56 kDa. (PMID: 27384875 )


Product Specific Protocols
WB protocol for YBX1 antibody 20339-1-APDownload protocol
IHC protocol for YBX1 antibody 20339-1-APDownload protocol
IF protocol for YBX1 antibody 20339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An

Sci Adv

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.

Authors - Chunxia Zhang

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
  • KD Validated

Cell Death Differ

YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer.

Authors - Dewei Jiang
  • KD Validated

Cell Rep

Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1.

Authors - Rosario Avolio
  • KD Validated

Nucleic Acids Res

YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.

Authors - Shawn M Lyons
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (03-11-2023)

Detects protein recruited to sodium arsenite induced stress granules (see image). 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / SH-SY5Y cells
YBX1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:20339-1-AP)

Sun (Verified Customer) (01-05-2023)

a good antibody. YBX1 is a quite abundant protein and this antibody detects it pretty well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T

Meliti (Verified Customer) (08-05-2020)

Not vey clean in terms of the background but gives a nice band corresponding to YBX1

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293
YBX1 Antibody Western Blot validation (1:3000 dilution) in HEK293 (Cat no:20339-1-AP)

H (Verified Customer) (04-06-2020)

This antibody works well in my hands using HEK293T cell lysates. I used LICOR anti-rabbit IRDye800 secondary antibody coupled with Odyssey Clx detection.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
YBX1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:20339-1-AP)