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YME1L1 Polyclonal antibody
YME1L1 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11510-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, HeLa cells, MCF-7 cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 13 publications below |
WB | See 54 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
IP | See 2 publications below |
Product Information
11510-1-AP targets YME1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | YME1L1 fusion protein Ag2069 |
Full Name | YME1-like 1 (S. cerevisiae) |
Calculated molecular weight | 716 aa, 80 kDa |
Observed molecular weight | 63 kDa |
GenBank accession number | BC023507 |
Gene symbol | YME1L1 |
Gene ID (NCBI) | 10730 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
Protocols
Product Specific Protocols | |
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WB protocol for YME1L1 antibody 11510-1-AP | Download protocol |
IF protocol for YME1L1 antibody 11510-1-AP | Download protocol |
IP protocol for YME1L1 antibody 11510-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
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Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
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Nat Cell Biol PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis. | ||
Mol Cell Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex
| ||
Mol Cell Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christopher (Verified Customer) (03-08-2022) | incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.
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