|Positive WB detected in||COLO 320 cells, HeLa cells, MCF-7 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11510-1-AP targets YME1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YME1L1 fusion protein Ag2069|
|Full Name||YME1-like 1 (S. cerevisiae)|
|Calculated molecular weight||716 aa, 80 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC023507|
|Gene ID (NCBI)||10730|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christopher (Verified Customer) (03-08-2022)
incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.