YME1L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YME1L1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

64

Reviews (1)

Cat no : 11510-1-AP

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

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Validation Data Gallery

• 

  WB analysis using 11510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11510-1-AP

  WB result of YME1L1 antibody (11510-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

• 

  WB analysis of HepG2 using 11510-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11510-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11510-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11510-1-AP

  IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IF Staining of HepG2 using 11510-1-AP

  Immunofluorescent analysis of HepG2 cells using 11510-1-AP (YME1L1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 13 publications below
• WB: See 54 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 2 publications below

Product Information

11510-1-AP targets YME1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YME1L1 fusion protein Ag2069
• Full Name: YME1-like 1 (S. cerevisiae)
• Calculated molecular weight: 716 aa, 80 kDa
• Observed molecular weight: 63 kDa
• GenBank accession number: BC023507
• Gene symbol: YME1L1
• Gene ID (NCBI): 10730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Publications

Species	Application	Title
mouse	WB	Cell Metab; C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.; Authors - Tao Wang ; KD Validated
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
mouse	WB	Mol Cell; Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex; Authors - Chen-Wei Tsai ; KD Validated
human	IF	Mol Cell; Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress.; Authors - Raul Jobava

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (03-08-2022)

incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

YME1L1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11510-1-AP)

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