Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11510-1-AP targets YME1L1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2069 Product name: Recombinant human YME1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 348-716 aa of BC023507 Sequence: GSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR Predict reactive species |
| Full Name | YME1-like 1 (S. cerevisiae) |
| Calculated Molecular Weight | 716 aa, 80 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC023507 |
| Gene Symbol | YME1L1 |
| Gene ID (NCBI) | 10730 |
| RRID | AB_2217459 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96TA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| IP protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| WB protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The YME1L1 Polyclonal antibody (Cat# 11510-1-AP) has accumulated 96 citations across 50+ journals, including Cell Metabolism, Nature Cell Biology, Nature Communications, Molecular Cell, EMBO Journal, Cell Reports, PNAS, and Cell Death & Differentiation. Research fields encompass mitochondrial dynamics and quality control, OPA1 proteolytic processing, cristae architecture, neurodegeneration, cancer biology, metabolic disorders, cardiomyopathy, renal disease, ferroptosis, and exercise physiology. Applications are predominantly Western blot, supplemented by IF, IHC, CoIP, and IP.
| Species | Application | Title |
|---|---|---|
Cell Metab Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.
| ||
Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. | ||
Cell Metab C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
| ||
Nat Cell Biol Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7 | ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
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Reviews
What customers say
Both reviews are for Western Blot applications. One user rated the antibody 5 stars, using it at 1:500 dilution on HTR cells with no additional comments. The other rated it 4 stars, using a 1:2000 dilution on 293T cells with a 1-hour room-temperature incubation. That user reported a weak signal at the expected molecular weight, noting the result was sensitive to a YME1L1-targeting sgRNA. Overall, the antibody performs as expected in WB, though signal strength may vary with dilution and cell type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vignesh (Verified Customer) (09-15-2025) |
|
Christopher (Verified Customer) (03-08-2022) | incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.
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