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  • KD/KO Validated

YME1L1 Polyclonal antibody

YME1L1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11510-1-AP

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11510-1-AP targets YME1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YME1L1 fusion protein Ag2069
Full Name YME1-like 1 (S. cerevisiae)
Calculated molecular weight 716 aa, 80 kDa
Observed molecular weight63 kDa
GenBank accession numberBC023507
Gene symbol YME1L1
Gene ID (NCBI) 10730
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Protocols

Product Specific Protocols
WB protocol for YME1L1 antibody 11510-1-APDownload protocol
IF protocol for YME1L1 antibody 11510-1-APDownload protocol
IP protocol for YME1L1 antibody 11510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.

Authors - Frank Richter
human

Mol Cell

Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.

Authors - Shiori Sekine
mouseWB

FEBS Open Bio

PMID: 30761259

Authors - Harumasa Nakazawa
humanWB

Nat Commun

ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Authors - Yuanyuan Zhao
human

EMBO Mol Med

Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.

Authors - Karthik Mohanraj
  • KD Validated
humanWB

J Cell Physiol

Laminar Shear Stress Promotes Mitochondrial Homeostasis in Endothelial Cells.

Authors - Li-Hong Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christopher (Verified Customer) (03-08-2022)

incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T
YME1L1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11510-1-AP)