|Positive WB detected in||COLO 320 cells, HeLa cells, MCF-7 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11510-1-AP targets YME1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YME1L1 fusion protein Ag2069|
|Full Name||YME1-like 1 (S. cerevisiae)|
|Calculated molecular weight||716 aa, 80 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC023507|
|Gene ID (NCBI)||10730|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
J Cell Biol
ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.
Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.
FEBS Open Bio
ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.
EMBO Mol Med
Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.
J Cell Physiol
Laminar Shear Stress Promotes Mitochondrial Homeostasis in Endothelial Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christopher (Verified Customer) (03-08-2022)
incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.