Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 14 publications below |
| WB | See 72 publications below |
| IHC | See 1 publications below |
| IF | See 9 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
11510-1-AP targets YME1L1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YME1L1 fusion protein Ag2069 Predict reactive species |
| Full Name | YME1-like 1 (S. cerevisiae) |
| Calculated Molecular Weight | 716 aa, 80 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC023507 |
| Gene Symbol | YME1L1 |
| Gene ID (NCBI) | 10730 |
| RRID | AB_2217459 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96TA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| IF protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| IP protocol for YME1L1 antibody 11510-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
| ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Nat Cell Biol PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis. | ||
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Mol Cell Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Christopher (Verified Customer) (03-08-2022) | incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.
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