ZC3HAV1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZC3HAV1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF

Conjugate

Unconjugated

Publications

22

Cat no : 16820-1-AP

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 16820-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16820-1-AP (ZC3HAV1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16820-1-AP

  IP Result of anti-ZC3HAV1 (IP:16820-1-AP, 4ug; Detection:16820-1-AP 1:2000) with HeLa cells lysate 1080ug.

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  IHC staining of human kidney using 16820-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16820-1-AP (ZC3HAV1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16820-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16820-1-AP (ZC3HAV1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 9 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 16820-1-AP is ZC3HAV1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, monkey, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3HAV1 fusion protein Ag10364
• Full Name: zinc finger CCCH-type, antiviral 1
• Calculated molecular weight: 699aa,78 kDa; 902aa,101 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC025308
• Gene symbol: ZC3HAV1
• Gene ID (NCBI): 56829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.

Publications

Species	Application	Title
	WB	J Virol; ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9.; Authors - Yongxiang Zhao ; KD Validated
human	IP	mBio; The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.; Authors - Ana Cristina Gonzalez-Perez
human,monkey	WB	Vet Microbiol; Porcine reproductive and respiratory syndrome virus Nsp4 cleaves ZAP to antagonize its antiviral activity.; Authors - Yongxiang Zhao
human, mouse	WB, IHC, IF	Antiviral Res; Zinc finger antiviral protein inhibits coxsackievirus B3 virus replication and protects against viral myocarditis.; Authors - Min Li
human	WB	Front Microbiol; Zinc Finger CCCH-Type Antiviral Protein 1 Restricts the Viral Replication by Positively Regulating Type I Interferon Response.; Authors - Baoge Zhang ; KD Validated
human	IF,RIP	mSphere; Association of Zinc Finger Antiviral Protein Binding to Viral Genomic RNA with Attenuation of Replication of Echovirus 7.; Authors - Niluka Goonawardane ; KO Validated

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