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ZC3HAV1 Antibody
ZC3HAV1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16820-1-AP
Synonyms
FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
The 16820-1-AP immunogen is Fusion Protein ZC3HAV1 protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZC3HAV1 fusion protein Ag10364 |
| Full Name | zinc finger CCCH-type, antiviral 1 |
| Calculated molecular weight | 699aa,78 kDa;902aa,101 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC025308 |
| Gene symbol | ZC3HAV1 |
| Gene ID (NCBI) | 56829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| IHC protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| IP protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9.
| ||
Antiviral Res Zinc finger antiviral protein inhibits coxsackievirus B3 virus replication and protects against viral myocarditis. | ||
Front Immunol CpG-Recoding in Zika Virus Genome Causes Host-Age-Dependent Attenuation of Infection With Protection Against Lethal Heterologous Challenge in Mice. | ||
PLoS Pathog Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.
| ||
BMC Biol RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. | ||
PLoS Pathog Inhibition of hepatitis B virus replication by the host zinc finger antiviral protein.
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