Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | rat stomach tissue, human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16820-1-AP targets ZC3HAV1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10364 Product name: Recombinant human ZC3HAV1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC025308 Sequence: MADPEVCCFITKILCAHGGRMALDALLQEIALSEPQLCEVLQVAGPDRFVVLETGGEAGITRSVVATTRARVCRRKYCQRPCDNLHLCKLNLLGRCNYSQSERNLCKYSHEVLSEENFKVLKNHELSGLNKEELAVLLLQSDPFFMPEICKSYKGEGRQQICNQQPPCSRLHICDHFTRGNCRFPNCLRSHNLMDRKVLAIMREHGLNPDVVQNIQDICNSKHMQKNPPGPRAPSSHRRNMAYRARSKSRDRFFQGSQEFLASASASAERSCTPSPDQISHRASLEDAPVDDLTRKFTYLGSQDRARPPSGSSKATDLGGTSQAGTSQRFLENGSQEDLLHGNPGSTYLAS Predict reactive species |
| Full Name | zinc finger CCCH-type, antiviral 1 |
| Calculated Molecular Weight | 699aa,78 kDa; 902aa,101 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC025308 |
| Gene Symbol | ZC3HAV1 |
| Gene ID (NCBI) | 56829 |
| RRID | AB_2728733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z2W4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| IP protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| WB protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZC3HAV1 (ZAP) Polyclonal antibody (Cat# 16820-1-AP) has 39 citations. These appear in high-impact journals including Nature, Science, Cell, Cell Host & Microbe, Nature Microbiology, Nature Communications, Nucleic Acids Research, EMBO Journal, PLoS Pathogens, PLoS Genetics, PLoS Biology, mBio, mSphere, eLife, and others. Research fields center on antiviral immunity and host–virus interactions (HIV, hepatitis B, Zika, poxviruses, SARS-CoV-2), RNA biology, retrotransposition, and inflammation/cancer.
| Species | Application | Title |
|---|---|---|
Nature CG dinucleotide suppression enables antiviral defence targeting non-self RNA.
| ||
Science Poxvirus attack of antiviral defense pathways unleashes an effector-triggered NF-κB response.
| ||
Cell Host Microbe HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization. | ||
Nat Microbiol FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins |
Reviews
What customers say
All four reviews rate the antibody 5 stars and use it for Western Blot. Users consistently report reliable, sharp signals even at high dilutions, with one noting it works well up to 1:20,000. Tested cell lines include HEK-293, HEK293T, U2OS, HELA, MT4, and SupT1. Overall, customers describe it as a fantastic, highly reliable antibody for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sven (Verified Customer) (08-05-2025) | I used the AB for WB, it worked very well.
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Lisa (Verified Customer) (08-05-2025) | Works perfectly for WB even highly diluted um to 1:20000
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Saul (Verified Customer) (07-23-2025) | really nice sharp signal even at quite high dilution
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Daniel (Verified Customer) (10-26-2022) | This is a fantastic antibody. It works very reliably at high dilutions.
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