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  • KD/KO Validated

ZC3HAV1 Polyclonal antibody

ZC3HAV1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16820-1-AP

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16820-1-AP targets ZC3HAV1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364
Full Name zinc finger CCCH-type, antiviral 1
Calculated molecular weight699aa,78 kDa; 902aa,101 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC025308
Gene symbol ZC3HAV1
Gene ID (NCBI) 56829
RRIDAB_2728733
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
IHC protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
IP protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CG dinucleotide suppression enables antiviral defence targeting non-self RNA.

Authors - Matthew A Takata
  • KD Validated
humanWB

Cell

SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.

Authors - Lea H Gregersen

Cell Host Microbe

HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.

Authors - Charles Bou-Nader
humanWB

Nat Microbiol

Rational attenuation of RNA viruses with zinc finger antiviral protein

Authors - Daniel Gonçalves-Carneiro
humanWB,IP

Nat Commun

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Authors - Matthias M Zimmer
humanWB

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (10-26-2022)

This is a fantastic antibody. It works very reliably at high dilutions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T, HELA, MT4, SupT1
ZC3HAV1 Antibody Western Blot validation (1:5000 dilution) in HEK293T, HELA, MT4, SupT1 (Cat no:16820-1-AP)