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ZC3HAV1 Antibody

ZC3HAV1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16820-1-AP

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 16820-1-AP immunogen is Fusion Protein ZC3HAV1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3HAV1 fusion protein Ag10364
Full Name zinc finger CCCH-type, antiviral 1
Calculated molecular weight 699aa,78 kDa;902aa,101 kDa
Observed molecular weight 100 kDa
GenBank accession number BC025308
Gene symbol ZC3HAV1
Gene ID (NCBI) 56829
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
IHC protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
IP protocol for ZC3HAV1 antibody 16820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Virol

ZAP, a CCCH-Type Zinc Finger Protein inhibits PRRSV replication and interacts with viral Nsp9.

Authors - Yongxiang Zhao
  • KD Validated
human, mouseWB, IHC, IF

Antiviral Res

Zinc finger antiviral protein inhibits coxsackievirus B3 virus replication and protects against viral myocarditis.

Authors - Min Li
mouseIHC

Front Immunol

CpG-Recoding in Zika Virus Genome Causes Host-Age-Dependent Attenuation of Infection With Protection Against Lethal Heterologous Challenge in Mice.

Authors - Ivan Trus
humanWB,IF

PLoS Pathog

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.

Authors - Hsin-Ping Chiu
  • KD Validated
humanWB

BMC Biol

RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.

Authors - Nila Roy Choudhury
humanWB

PLoS Pathog

Inhibition of hepatitis B virus replication by the host zinc finger antiviral protein.

Authors - Mao Richeng R
  • KD Validated