|Positive WB detected in||MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18980-1-AP targets ZCCHC11 in WB, IP, IF, CoIP,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||zinc finger, CCHC domain containing 11|
|Calculated molecular weight||185 kDa|
|Observed molecular weight||185 kDa|
|GenBank accession number||NM_015269|
|Gene ID (NCBI)||23318|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can’t bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis.
Nat Struct Mol Biol
Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance.
Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.
MiRAR-miRNA Activity Reporter for Living Cells.
3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.
Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.