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- KD/KO Validated
ZCCHC11 Polyclonal antibody
ZCCHC11 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IF, CoIP, ELISA
Cat no : 18980-1-AP
Validation Data Gallery
|Positive WB detected in||MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 10 publications below|
|WB||See 22 publications below|
|IF||See 3 publications below|
|IP||See 1 publications below|
|CoIP||See 2 publications below|
18980-1-AP targets ZCCHC11 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||zinc finger, CCHC domain containing 11|
|Calculated molecular weight||185 kDa|
|Observed molecular weight||185 kDa|
|GenBank accession number||NM_015269|
|Gene ID (NCBI)||23318|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
|Product Specific Protocols|
|WB protocol for ZCCHC11 antibody 18980-1-AP||Download protocol|
|IF protocol for ZCCHC11 antibody 18980-1-AP||Download protocol|
|IP protocol for ZCCHC11 antibody 18980-1-AP||Download protocol|
|Click here to view our Standard Protocols|
mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.
A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.
Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.
3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
A Mechanism for microRNA Arm Switching Regulated by Uridylation.