Validation Data Gallery
|Positive WB detected in
|MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue
|Positive IP detected in
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18980-1-AP targets ZCCHC11 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|zinc finger, CCHC domain containing 11
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.
A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.
Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.
3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
A Mechanism for microRNA Arm Switching Regulated by Uridylation.