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  • KD/KO Validated

ZCCHC11 Polyclonal antibody

ZCCHC11 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 18980-1-AP

Synonyms

KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11



Tested Applications

Positive WB detected inMCF7 cells, HEK-293T cells, HeLa cells, human brain tissue
Positive IP detected inMCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18980-1-AP targets ZCCHC11 in WB, IP, IF, CoIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name zinc finger, CCHC domain containing 11
Calculated molecular weight 185 kDa
Observed molecular weight 185 kDa
GenBank accession numberNM_015269
Gene symbol ZCCHC11
Gene ID (NCBI) 23318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.

Protocols

Product Specific Protocols
WB protocol for ZCCHC11 antibody 18980-1-APDownload protocol
IF protocol for ZCCHC11 antibody 18980-1-APDownload protocol
IP protocol for ZCCHC11 antibody 18980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Res

A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis.

Authors - Marcos Morgan
  • KO Validated
mouseWB

Nat Struct Mol Biol

Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance.

Authors - Mehdi Pirouz
humanWB

Cell Rep

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.

Authors - Kazutoshi Takahashi
humanWB

Genes (Basel)

MiRAR-miRNA Activity Reporter for Living Cells.

Authors - Matthew A Turk
  • KD Validated
humanIF

RNA

3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.

Authors - Cristina Gutiérrez-Vázquez
mouseWB

Sci Rep

Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.

Authors - Silvia Parisi