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  • KD/KO Validated

ZCCHC11 Polyclonal antibody

ZCCHC11 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18980-1-AP

Synonyms

KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11



Tested Applications

Positive WB detected inMCF7 cells, HEK-293T cells, HeLa cells, human brain tissue
Positive IP detected inMCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18980-1-AP targets ZCCHC11 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name zinc finger, CCHC domain containing 11
Calculated molecular weight 185 kDa
Observed molecular weight 185 kDa
GenBank accession numberNM_015269
Gene symbol ZCCHC11
Gene ID (NCBI) 23318
RRIDAB_10598327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.

Protocols

Product Specific Protocols
WB protocol for ZCCHC11 antibody 18980-1-APDownload protocol
IF protocol for ZCCHC11 antibody 18980-1-APDownload protocol
IP protocol for ZCCHC11 antibody 18980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.

Authors - Marcos Morgan
  • KO Validated
mouseWB

Nature

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.

Authors - Chang Hao-Ming HM
  • KD Validated
humanWB

Cell

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.

Authors - Piskounova Elena E
  • KD Validated
humanWB,IF

Cell

Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.

Authors - Zbigniew Warkocki
humanWB

Mol Cell

3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.

Authors - Acong Yang
  • KO Validated
humanWB

Mol Cell

A Mechanism for microRNA Arm Switching Regulated by Uridylation.

Authors - Haedong Kim
  • KD Validated