Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human gliomas tissue, human lung cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14026-1-AP targets ZEB2 in WB, IHC, IF, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5171 Product name: Recombinant human ZEB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC060819 Sequence: MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPGKCLKSLDLFLYHLHIFN Predict reactive species |
| Full Name | zinc finger E-box binding homeobox 2 |
| Calculated Molecular Weight | 136 kDa |
| Observed Molecular Weight | 150-170 kDa |
| GenBank Accession Number | BC060819 |
| Gene Symbol | ZEB2 |
| Gene ID (NCBI) | 9839 |
| RRID | AB_2878001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZEB2 antibody 14026-1-AP | Download protocol |
| WB protocol for ZEB2 antibody 14026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ZEB2 Polyclonal antibody (Cat# 14026-1-AP) has 92 citations published in journals including Nature Metabolism, Nature Cell Biology, Nature Communications, Theranostics, Cell Death & Differentiation, and Journal of Experimental & Clinical Cancer Research. Research fields predominantly cover epithelial-mesenchymal transition (EMT), tumor metastasis, renal fibrosis, and wound healing, with cited applications spanning WB, IF, IHC, ChIP, CoIP, and RIP.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection
| ||
Nat Commun Conserved regulatory switches for the transition from natal down to juvenile feather in birds | ||
Nat Commun Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. | ||
J Adv Res OTUD7B attenuates renal fibrosis by regulating PRDX1 protein stability.
| ||
Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. |
