ZNF644 Polyclonal antibody

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  WB analysis using 25956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25956-1-AP (ZNF644 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ZNF644 Polyclonal Antibody for WB, ELISA

Cat No. 25956-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZEP2, Zep 2

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Tested Applications

• Positive WB detected in: Y79 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25956-1-AP targets ZNF644 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF644 fusion protein Ag22798
• Full Name: zinc finger protein 644
• Calculated Molecular Weight: 1327 aa, 150 kDa
• Observed Molecular Weight: 175 kDa
• GenBank Accession Number: BC063683
• Gene Symbol: ZNF644
• Gene ID (NCBI): 84146
• RRID: AB_3669512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H582
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G9a/GLP complex is associated with Polycomb Repressive Complex 2 (PRC2).The G9a/GLP complex also regulates DNA methylation during early embryogenesis, which is independent of their methyltransferase activity. ZNF644 and WIZ, two zinc finger proteins, interact with G9a and GLP, respectively. These two zinc finger proteins target G9a and GLP to genomic loci for the regulation of gene transcription.