ZSCAN2 Polyclonal antibody

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  WB analysis using 32581-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32581-1-AP (ZSCAN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ZSCAN2 Polyclonal Antibody for WB, ELISA

Cat No. 32581-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNF854, Zinc finger protein 854, Zinc finger protein 29 homolog, Zinc finger and SCAN domain-containing protein 2, Zfp-29

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Tested Applications

• Positive WB detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32581-1-AP targets ZSCAN2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZSCAN2 fusion protein Ag38126
• Full Name: zinc finger and SCAN domain containing 2
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC136342
• Gene Symbol: ZSCAN2
• Gene ID (NCBI): 54993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q7Z7L9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger and SCAN domain-containing protein 2 (ZSCAN2, also known as ZFP29 and ZNF854) plays an important role in the maintenance of the SSC (spermatogonial stem cells) pool in vitro and is integral for robust regeneration of spermatogenesis in vivo following exposure to clastogens. Loss of ZSCAN2 expression alters key cellular processes in undifferentiated spermatogonia, such as translation, chromatin modification, and ubiquitin conjugation (PMID: 35244684).